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TABLE 1.
Inactivation of HSV-2 infectivity by SDS
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| % SDS
during treatmenta |
Final % SDSb |
No. of
plaques/platec
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|
| 0 |
0 |
57,
73, 343, 145, 145 |
1 × 10 1 |
1 × 103 |
0,
0, 0, 0, 0 |
| 5 × 102 |
5 × 104 |
0, 0, 0, 0, 0 |
| 2.5 × 102 |
2.5 × 104 |
0, 0, 0, 0, 0 |
| 1.25 × 102 |
1.25 × 104 |
0, 0, 0, 2, 81 |
| 2.5 × 103 |
2.5 × 105 |
28, 54, 322, 145, 104 |
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a
Sterile SDS stocks (40 times the
treatment concentration) were added to virus aliquots to achieve the
treatment concentration. After mixing, the samples were incubated at
37°C for 10 min.
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b
Following SDS treatment, virus stocks were
diluted 100-fold and 1-ml aliquots were immediately adsorbed onto CV-1
cells. Plaques were counted after 20 to 24 h of infection. Each
number represents an average for two plates.
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c
Data are values from five experiments.
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