|
|
TABLE 3.
Inhibition of BPV-1 morphologic transformation of C127
cells by SDS treatment of virus
|
|
| % SDS during
treatmenta |
Final % SDSb |
No. of
foci/platec
|
| Expt 1 (day
12) |
Expt 2
|
| Day 14 |
Day 17 |
|
| 0 |
0 |
266 |
255 |
153
|
| 5 |
5 × 10 3 |
0 |
ND |
ND
|
5 × 10 1 |
5 × 10 4 |
0 |
ND |
ND
|
5 × 10 2 |
5 × 10 5 |
0 |
0 |
0
|
5 × 10 3 |
5 × 10 6 |
0 |
271 |
150
|
2.5 × 10 3 |
2.5 × 10 6 |
ND |
273 |
162
|
5 × 10 4 |
5 × 10 7 |
ND |
229 |
151 |
|
|
a
Sterile SDS stocks (40 times the
treatment concentration) were added to virus aliquots to achieve the
treatment concentration.
|
|
b
Following virus treatment, treated virus
stocks were further diluted 1:1,000 in order to dilute the detergent;
the cells in all plates remained viable.
|
|
c
In experiment 1, virus and SDS were mixed and
incubated at 37°C for 30 min; in experiment 2, virus and SDS were
mixed and incubated at 37°C for 10 min. ND, not done. In control
plates without BPV-1, no foci appeared.
|
|