|
|
TABLE 2.
Inhibition of replication of avian influenza A viruses in
MDCK cells by RWJ-270201, zanamivir, and oseltamivir carboxylate
|
|
| NA
subtype |
Virusb |
Mean
EC50 ± SE (µM)a
|
| RWJ-270201 |
Zanamivir |
Oseltamivir carboxylate
|
|
| N1 |
A/teal/Hong Kong/W312/97
(H6N1) |
5.0 ± 1.8 |
9.4 ± 0.7 |
8.1 ± 1.6
|
|
A/duck/Alberta/35/76
(H1N1) |
1.1 ± 0.4 |
11.3 ± 2.4 |
11.9 ± 0.5
|
| N2 |
A/chicken/NY/13307-3/95
(H7N2) |
1.1 ± 0.3 |
6.9 ± 0.8 |
1.0 ± 0.3
|
|
A/turkey/Wisconsin/66
(H9N2) |
0.9 ± 0.4 |
19.7 ± 0.9 |
1.0 ± 0.1
|
| N3 |
A/duck/Singapore/3/97
(H5N3) |
1.0 ± 0.5 |
10.3 ± 0.2 |
1.6 ± 0.5
|
|
A/duck/Germany/1215/73
(H2N3) |
1.4 ± 0.2 |
32.3 ± 1.5 |
6.8 ± 1.2
|
| N4 |
A/red knot/DE/254/94
(H8N4) |
8.5 ± 2.5 |
17.5 ± 0.5 |
17.9 ± 0.1
|
|
A/turkey/Ontario/6118/68
(H8N4) |
2.6 ± 1.4 |
12.0 ± 1.8 |
6.2 ± 2.2
|
| N5 |
A/mallard duck/Astrakhan/263/82
(H14N5) |
11.8 ± 0.5 |
12.0 ± 0.3 |
13.0 ± 1.0
|
|
A/shearwater/Australia/1/72
(H6N5) |
2.7 ± 0.9 |
19.3 ± 3.0 |
3.5 ± 1.4
|
| N6 |
A/shorebird/DE/224/97
(H13N6) |
11.0 ± 0.8 |
11.2 ± 0.6 |
10.2 ± 0.6
|
|
A/duck/Czechoslovakia/56
(H4N6) |
8.7 ± 1.7 |
42.0 ± 1.5 |
10.7 ± 0.9
|
| N7 |
A/chicken/Jena/1816/87
(H7N7) |
0.5 ± 0.1 |
12.3 ± 1.5 |
4.7 ± 0.9
|
|
A/duck/Germany/"N"/49
(H10N7) |
1.7 ± 0.5 |
27.0 ± 1.2 |
3.8 ± 0.8
|
| N8 |
A/duck/Hong Kong/Y264/97
(H4N8) |
2.3 ± 0.8 |
4.0 ± 1.0 |
3.5 ± 2.5
|
|
A/quail/Italy/1117/65
(H10N8) |
2.7 ± 0.4 |
55.0 ± 8.8 |
1.0 ± 0.1
|
| N9 |
A/duck/Hong Kong/P50/97
(H11N9) |
7.9 ± 1.7 |
10.8 ± 0.6 |
3.7 ± 3.3
|
|
A/duck/Memphis/546/74
(H11N9) |
11.7 ± 1.8 |
58.3 ± 1.7 |
42.0 ± 1.5
|
| Range (N1-N9) |
Range of
values |
0.5-11.8 |
4.0-58.3 |
1.0-42.0 |
|
|
a
Antiviral activity was assessed in MDCK
cells by a modified microneutralization assay followed by ELISA. Values
are means ± SEs for at least three independent determinations.
|
|
b
The second strain in each pair is the
reference strain of influenza A virus used for the antigenic subtyping
of the NA of the new influenza isolates.
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