TABLE 1. Distribution of protein and ATPase in fractions of dialyzed membranesa

Membrane Fraction ATPase

U/mg of protein Total U

Control Depleted membrane 0.036 2.3
Concentrated supernatant 0.134 4.82
E1 treated Depleted membrane 0.034 1.98
Concentrated supernatant 0.11 4.6

a Specific activities of ATPase of nondepleted membranes from control and treated bacteria were 0.21 and 0.20, respectively.