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Antimicrob Agents Chemother. 1972 January; 1(1): 22-29
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Transferable Drug Resistance in Pseudomonas aeruginosa¹

^a Department of Medical Bacteriology, University of Alberta, Edmonton, Alberta, Canada

ABSTRACT

Three strains of Pseudomonas aeruginosa were demonstrated to transfer double-drug resistance by conjugation to a P. aeruginosa recipient at frequencies of 10^–4 to 10^–2 per recipient cell. Two of the three strains also transferred to Escherichia coli at frequencies which were 10³- to 10⁵-fold lower, but the third strain could not be demonstrated to do so. The latter strain, however, conferred maleness on the Pseudomonas recipient. The transfer of streptomycin resistance was associated with the acquisition of streptomycin phosphorylase by both P. aeruginosa and E. coli recipients. Maximal broth mating frequencies were obtained with nonagitated cultures less than 1 mm in depth. A pyocine selection system based on donor sensitivity and recipient resistance is described and appears to have future value as a generalized selective device for use after matings.

¹ Presented in part at the 71st Annual Meeting of the American Society for Microbiology, Minn., 2–7 May 1971.

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