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Antimicrob Agents Chemother. 1972 February; 1(2): 139-142
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Antibacterial Activity of Lividomycin Toward R Factor-Resistant Strains of Escherichia coli

^a Department of Microbiology, School of Medicine, Gunma University, Maebashi, Japan

ABSTRACT

Forty-eight R factors conferring resistance to both kanamycin (KM) and streptomycin (SM) were demonstrated from 1,270 Escherichia coli strains of clinical origin. Among these R factors isolated, 42 also conferred resistance to the new antibiotic lividomycin (LV). Extracts of E. coli ML1410 carrying one such factor (R_M81) inactivated LV as well as KM and SM. But extracts of E. coli ML1410 carrying an R factor (R_M82 or R_M83) sensitive to LV could not inactivate LV. LV inactivation required both adenosinetriphosphate (ATP) and Mg²⁺. Inactivated LV was reactivable by alkaline phosphatase. Isotopic studies with labeled ATP and elemental analysis of the reaction product indicate that it is a monophosphorylated derivative of LV.

¹ Present address: Tokyo Research Laboratories, Kowa Co., Ltd., Higashimurayama, Tokyo.