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Antimicrob Agents Chemother. 1972 June; 1(6): 451-459
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Standardized Single-Disc Method for Antibiotic Susceptibility Testing of Anaerobic Bacteria

^a Anaerobe Laboratory, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24060

ABSTRACT

A method was developed for determination of the antibiotic susceptibility of anaerobic bacteria by use of a single-disc diffusion technique and incorporation of the inoculum in pour plates. The method was standardized by correlation of zone diameters with minimal inhibitory concentrations determined in broth. Zone diameters could be used to approximate the minimal inhibitory concentrations of the seven antibiotics tested: ampicillin, bacitracin, carbenicillin, cephalothin, clindamycin, penicillin, and tetracycline.

¹ Present address: National Cancer Institute, Baltimore Cancer Research Center, Baltimore, Md. 21211.