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Antimicrob Agents Chemother. 1977 January; 11(1): 13-20
Copyright © 1977 American Society for Microbiology. All Rights Reserved.

Temperature-Sensitive R Plasmid Obtained from Naturally Isolated Drug-Resistant Vibrio cholerae (Biotype El Tor)

^* Department of Bacteriology, School of Medicine, Juntendo University, Tokyo 113, Japan
¹ Department of Microbiology, School of Medicine, Toho University, Tokyo 143, Japan

ABSTRACT

A temperature-sensitive fi^– R plasmid, pJY1, which confers resistance to chloramphenicol, streptomycin, and sulfonamide, was found in a drug-resistant strain of Vibrio cholerae (biotype El Tor) isolated in the Philippines in 1973. The R plasmid is temperature sensitive for transferability but not for stability in the hosts. pJY1 is stably maintained either in V. cholerae or in Escherichia coli at a range of culture temperatures from 27 to 42°C. Compatibility tests with reference R plasmids revealed that pJY1 belongs to the J group. Growth of V. cholerae carrying pJY1 is poor on TCBS (thiosulfate-citrate-bile-sucrose) selective plates when the microbe is subcultured in nutrient broth at temperatures higher than 37°C.

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