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Antimicrob Agents Chemother. 1977 January; 11(1): 31-33
Copyright © 1977 American Society for Microbiology. All Rights Reserved.

Bioassay Method for Polyene Antibiotics Based on the Measurement of Rubidium Efflux from Rubidium-Loaded Yeast Cells

Squibb International Development Laboratory, Moreton, Wirral, Merseyside L46 IQW, England

ABSTRACT

A bioassay method for the polyene antibiotics nystatin and amphotericin B is proposed based on the measurement of the efflux of rubidium ions from a rubidium-loaded yeast culture challenged with the antibiotics. For this purpose a major proportion of the intracellular K⁺ ions in a Saccharomyces cerevisiae culture has been substituted by Rb⁺ ions. The rubidium leakage is measured by atomic absorption spectrophotometry, and a straight-line, dose-response correlation has been obtained for both antibiotics.