This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Williams, W. K. Articles by Katz, E. Search for Related Content PubMed PubMed Citation Articles by Williams, W. K. Articles by Katz, E.

 Previous Article  |  Next Article 

Antimicrob Agents Chemother. 1977 February; 11(2): 281-290
Copyright © 1977 American Society for Microbiology. All Rights Reserved.

Development of a Chemically Defined Medium for the Synthesis of Actinomycin D by Streptomyces parvulus

Department of Microbiology, Georgetown University Schools of Medicine and Dentistry, Washington, D. C. 20007

ABSTRACT

A chemically defined medium, consisting of D-fructose, L-glutamic acid, L-histidine, K₂HPO₄, MgSO₄·7H₂O, ZnSO₄·7H₂O, CaCl₂·2H₂O, FeSO₄·7H₂O, CoCl₂·6H₂O, and deionized water, was developed for synthesis of high yields (500 to 600 µg/ml) of actinomycin D by Streptomyces parvulus. Under these nutritional conditions, growth and actinomycin formation did not follow a typical trophophase-idiophase pattern. The amino acids appeared to have a sparing action on the utilization of D-fructose which was slowly and incompletely metabolized during mycelium development and antibiotic production. A significant repression of actinomycin synthesis by S. parvulus was observed when D-glucose (0.01 to 0.25%) was added to the culture medium. The repression was not due to a decline in the pH of the medium during glucose catabolism.

This article has been cited by other articles:

• Voelker, F., Altaba, S. (2001). Nitrogen source governs the patterns of growth and pristinamycin production in 'Streptomyces pristinaespiralis'. Microbiology 147: 2447-2459 [Abstract] [Full Text]