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Antimicrob Agents Chemother. 1977 March; 11(3): 482-490
Copyright © 1977 American Society for Microbiology. All Rights Reserved.

Comparative Growth Rates of Selected Anaerobic Species in Four Commonly Used Broth Media

¹ Microbiology Research Laboratory, Department of Pathology, Mount Sinai Medical Center, Milwaukee, Wisconsin 53201

ABSTRACT

Generations times (g_t) of selected anaerobic species were compared in four culture media commonly used for anaerobic bacteria to evaluate these media for growing inocula for anaerobic antibiotic susceptibility determinations. Twenty-two clinical isolates of Bacteroides fragilis, Fusobacterium spp., Clostridium perfringens, and Clostridium spp. were tested in Schaedler broth, supplemented Lombard-Dowell broth, supplemented thioglycolate broth, and supplemented peptone-yeast extract-glucose broth. Growth curves were performed in an anaerobic chamber; changes in cell density were followed by viable count procedures and, in some cases, turbidometric procedures. The clostridia did not appear to have significantly different g_t values in different media. The g_t values for the five clostridial species tested ranged from 18 to 45 min. The fusobacteria also showed no apparent differences in g_t values in the four media; values ranged from 51 to 60 min. The two subspecies of B. fragilis tested showed no significant differences in g_t values when compared with each other in the same media. The combined data, however, indicated that the growth rate in Schaedler broth was faster than in the other media (average g_t, 45.1 min), whereas the growth rate in peptone-yeast extract-glucose was slower (average g_t, 58.2 min). The growth rates in supplemented thioglycolate and supplemented Lombard-Dowell broth (average g_t, 53.6 min and average g_t, 51.0 min, respectively) were not significantly different.

¹ Present address: Department of Pathology, Chicago Osteopathic Hospital, Chicago, IL 60615.