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Antimicrob Agents Chemother. 1977 April; 11(4): 651-655
Copyright © 1977 American Society for Microbiology. All Rights Reserved.

Assay of Gentamicin in Serum by High-Pressure Liquid Chromatography

John P. Anhalt

1 Department of Laboratory Medicine, Mayo Clinic and Mayo Foundation, Rochester, Minnesota 55901

ABSTRACT

A procedure is described that uses high-pressure liquid chromatography for assay of gentamicin in serum. The technique involves extraction of gentamicin from serum by using a CM-Sephadex column and analysis by reverse-phase, ion-pair chromatography. Continuous-flow, post-column derivatization with o-phthaladehyde is used to form fluorescent products for detection. The possible scope of this method for analysis of other aminoglycoside antibiotics is illustrated by application to sisomicin and netilmicin.

Antimicrob Agents Chemother. 1977 April; 11(4): 651-655
Copyright © 1977 American Society for Microbiology. All Rights Reserved.





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Copyright © 1977 by the American Society for Microbiology. All rights reserved.