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Antimicrob Agents Chemother. 1977 June; 11(6): 1074-1076
Copyright © 1977 American Society for Microbiology. All Rights Reserved.

Double-Osmotic-Shock Procedure to Prepare Gentamicin Adenine Mononucleotide Transferase for the Enzymatic Assay of Gentamicin

J. Dankert and J. Woltjes

Laboratory of Medical Microbiology, Division of Hospital Infections, University of Groningen, A.Z.G., Groningen, The Netherlands

ABSTRACT

The release of gentamicin adenine mononucleotide transferase during single, cold, osmotic-shock treatment of Escherichia coli K-12 W677/HJR66 is not always maximal. The quantity of gentamicin adenine mononucleotide transferase in osmotic extracts could be significantly increased when a double-shock procedure was performed.


Antimicrob Agents Chemother. 1977 June; 11(6): 1074-1076
Copyright © 1977 American Society for Microbiology. All Rights Reserved.







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Copyright © 1977 by the American Society for Microbiology. All rights reserved.