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Antimicrob Agents Chemother. 1977 June; 11(6): 993-998
Copyright © 1977 American Society for Microbiology. All Rights Reserved.

Evaluation of Three Newer Methods for Investigating Protein Interactions of Penicillin G

¹ Infectious Disease Section, Department of Medicine, Minneapolis Veterans Administration Hospital; and University of Minnesota Medical School, Minneapolis, Minnesota 55417

ABSTRACT

The interaction of penicillin G with human serum proteins was evaluated by three different techniques: rate of dialysis, cross-linked dextran exclusion, and ultracentrifugation. The rate-of-dialysis technique demonstrated that penicillin G binding to serum was immediate but incompletely reversible. Cross-linked dextran adsorbed or trapped significant amounts of penicillin G, necessitating correction factors of more than 10%. Ultracentrifugation was found to be the most reliable method for quantitative protein-binding determinations of penicillins.

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