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Antimicrob Agents Chemother. 1977 July; 12(1): 11-15
Copyright © 1977 American Society for Microbiology. All Rights Reserved.

Human Interferon: Mass Production in a Newly Established Cell Line, MG-63

University of Leuven, Department of Human Biology, Rega Institute for Medical Research, B-3000 Leuven, Belgium

ABSTRACT

MG-63 cells, a line derived from an osteosarcoma, produced high yields of interferon after superinduction with polyinosinic acid·polycytidylic acid, cycloheximide, and actinomycin D. Advantages of MG-63 cells over diploid fibroblasts as a substrate are: no requirement for aging between confluency and induction, no requirement for priming, and 3.7-fold higher yields per square centimeter of culture surface. Physicochemically and biologically, MG-63 cell interferon resembles fibroblast rather than leukocyte interferon.

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