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Antimicrob Agents Chemother. 1977 September; 12(3): 410-417
Copyright © 1977 American Society for Microbiology. All Rights Reserved.

Bacteriocin from Actinomyces odontolyticus with Temperature-Dependent Killing Properties

Colin K. Franker, C. Achille Herbert and Stuart Ueda

Section of Oral Biology and Dental Research Institute, School of Dentistry, University of California, Los Angeles, California 90024

ABSTRACT

A strain of Actinomyces odontolyticus, originally isolated from human dental plaque, produced a non-dialyzable, trypsin-sensitive substance that was bactericidal for certain strains of bifidobacteria at 42°C but not at 37°C. Detectable quantities of the bacteriocin were not produced in liquid media. Experimentally useful yields were obtained by extraction from pour plate cultures of producer cells. At 42°C, exponential killing did not occur until indicator cells had doubled at least once. At 37°C, the bacteriocin effected a transient bacteriostasis. Partially purified concentrates were obtained by diethylaminoethyl-cellulose chromatography, and such material was not inactivated by ribonuclease, deoxyribonuclease, or lipase. Pronase, trypsin, and exposure to 100°C for 20 min completely abolished activity. Inhibitory activity was considerably reduced by exposure to a pH of either 3 or 11. Treatment of producer cells with curing agents did not induce a high frequency of non-bacteriocinogenic cells. The odontolyticin was adsorbed by susceptible, as well as resistant, bacteria.

Antimicrob Agents Chemother. 1977 September; 12(3): 410-417
Copyright © 1977 American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Tompkins, G.R., Tagg, J.R. (1986). Incidence and Characterization of Anti-microbial Effects Produced by Actinomyces viscosus and Actinomyces naeslundii. JDR 65: 109-112 [Abstract]  
  • Turner, J.W., Jordan, H.V. (1981). Basic Biological Sciences: Bacteriocin-like Activity Within the Genus Actinomyces. JDR 60: 1000-1007 [Abstract]  


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