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Antimicrob Agents Chemother. 1979 August; 16(2): 210-213
Copyright © 1979, American Society for Microbiology. All Rights Reserved.

New Rapid Assay for Nafcillin in Serum by Spectrofluorometry

Diane M. Lichtenwalner, Byungse Suh, Bennett Lorber and Alan M. Sugar

1 Section of Infectious Diseases, Temple University Health Sciences Center, Philadelphia, Pennsylvania 19140

ABSTRACT

A new, rapid method for measuring serum levels of nafcillin by spectrofluorometry is described. The method involves extraction of 2 ml of acidified serum with n-butyl chloride, subjecting the organic solvent layer to excitation at 340 nm, and measuring the relative intensity of emission fluorescence at 380 nm. An excellent linear correlation exists between serum levels of nafcillin and the relative intensity in a drug concentration range of 0.25 to 150 µg/ml. The results obtained by this spectrofluorometric technique are in complete accord with those obtained by the conventional microbiological assay using Staphylococcus aureus ATCC 6538P. The method is not interfered with by elevated levels of endogenous metabolic products or the presence of other drugs, including a number of antimicrobial agents. The assay is interfered with, however, by the presence of salicylates, for which appropriate correction can easily be made. A salicylate assay employing a spectrofluorometric technique is also described.

Antimicrob Agents Chemother. 1979 August; 16(2): 210-213
Copyright © 1979, American Society for Microbiology. All Rights Reserved.





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