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Antimicrob Agents Chemother. 1979 August; 16(2): 221-224
Copyright © 1979, American Society for Microbiology. All Rights Reserved.

Spectrophotometric Assay for Amikacin Using Purified Kanamycin Acetyltransferase

Emanual Scarbrough, Jeffrey W. Williams and Dexter B. Northrop

School of Pharmacy, University of Wisconsin, Madison, Wisconsin 53706

ABSTRACT

A rapid spectrophotometric assay has been developed for measuring the concentrations of amikacin and related antibiotics in serum. The assay uses a purified enzyme from R-factor E. coli which acetylates amikacin with the production of coenzyme A, the latter in turn being reacted with a sulfhydryl reagent to produce stoichiometric amounts of a sensitive chromophore, that is measured in the visible spectrum. The system complements an earlier assay for gentamicin-related antibiotics thereby facilitating the rapid measurement of the concentrations of all clinically important aminoglycosides in serum.

Antimicrob Agents Chemother. 1979 August; 16(2): 221-224
Copyright © 1979, American Society for Microbiology. All Rights Reserved.





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