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Antimicrob Agents Chemother. 1980 October; 18(4): 506-510

Enzymatic method for rapid and sensitive determination of beta-lactam antibiotics.

J M Frère, D Klein and J M Ghuysen

ABSTRACT

A rapid and sensitive procedure for the estimation of beta-lactam antibiotics is described which makes use of the ability of these antibiotics to inactivate the R39 DD-carboxypeptidase. Depending on the values of the kinetic parameters which govern the reaction, the antibiotics fall into two groups. The lower limit for the quantitative estimation of the antibiotics of groups I and II is about 5 and 50 pmol/ml, respectively. The procedure has been adopted to biological fluids such as human sera and cows' milk.

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Antimicrob Agents Chemother. 1980 October; 18(4): 506-510

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