This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Foulstone, M Articles by Reading, C Search for Related Content PubMed PubMed Citation Articles by Foulstone, M Articles by Reading, C

 Previous Article  |  Next Article 

Antimicrob Agents Chemother. 1982 November; 22(5): 753-762

Assay of amoxicillin and clavulanic acid, the components of Augmentin, in biological fluids with high-performance liquid chromatography.

M Foulstone and C Reading

ABSTRACT

Augmentin is a new antibacterial formulation comprised of amoxicillin and the beta-lactamase inhibitor clavulanic acid. In the present paper, the use of high-performance liquid chromatography (HPLC) to provide a rapid assay of the components of Augmentin in body fluids is described. Clavulanic acid was assayed by reacting the sample with imidazole, which readily produces a derivative absorbing at 311 nm. This derivative chromatographs on reverse-phase HPLC columns clear of interfering components in both human serum and urine. Concentrations of clavulanic acid as low as 0.1 microgram/ml were readily detectable in human serum with this procedure. There was no interference from amoxicillin, amoxicillin penicilloic acid, or the acid and alkali degradation products of clavulanic acid when this assay system was used. Amoxicillin in body fluids was assayed directly by HPLC without derivatization. The same chromatographic conditions were employed for the assay of amoxicillin and the clavulanic acid derivative, simplifying the methodology. Amoxicillin, however, was determined of the antibiotic per ml. An alkali blanking procedure for amoxicillin and clavulanic acid is also described which allows the detection of any underlying peaks which may cochromatograph. The use of ultrafiltration to remove protein from serum samples before HPLC was successfully applied to the assay of clavulanic acid and amoxicillin. Ultrafiltration is not an essential procedure for these assays, but it prolongs column life and reduces interference in the amoxicillin assay. Results obtained by HPLC were compared with those obtained by using microbiological assays.

---

Antimicrob Agents Chemother. 1982 November; 22(5): 753-762

This article has been cited by other articles:

• Arulanantham, H., Kershaw, N. J., Hewitson, K. S., Hughes, C. E., Thirkettle, J. E., Schofield, C. J. (2006). ORF17 from the Clavulanic Acid Biosynthesis Gene Cluster Catalyzes the ATP-dependent Formation of N-Glycyl-clavaminic Acid. J. Biol. Chem. 281: 279-287 [Abstract] [Full Text]  
• de la Fuente, A., Martin, J. F., Rodriguez-Garcia, A., Liras, P. (2004). Two Proteins with Ornithine Acetyltransferase Activity Show Different Functions in Streptomyces clavuligerus: Oat2 Modulates Clavulanic Acid Biosynthesis in Response to Arginine. J. Bacteriol. 186: 6501-6507 [Abstract] [Full Text]  
• Jin, W., Ryu, Y. G., Kang, S. G., Kim, S. K., Saito, N., Ochi, K., Lee, S. H., Lee, K. J. (2004). Two relA/spoT homologous genes are involved in the morphological and physiological differentiation of Streptomyces clavuligerus. Microbiology 150: 1485-1493 [Abstract] [Full Text]  
• Cremieux, A.-C., Muller-Serieys, C., Panhard, X., Delatour, F., Tchimichkian, M., Mentre, F., Andremont, A. (2003). Emergence of Resistance in Normal Human Aerobic Commensal Flora during Telithromycin and Amoxicillin-Clavulanic Acid Treatments. Antimicrob. Agents Chemother. 47: 2030-2035 [Abstract] [Full Text]  
• Fonseca, W., Hoppu, K., Rey, L. C., Amaral, J., Qazi, S. (2003). Comparing Pharmacokinetics of Amoxicillin Given Twice or Three Times per Day to Children Older than 3 Months with Pneumonia. Antimicrob. Agents Chemother. 47: 997-1001 [Abstract] [Full Text]  
• Burkhardt, O., Borner, K., von der Hoh, N., Koppe, P., Pletz, M. W., Nord, C. E., Lode, H. (2002). Single- and multiple-dose pharmacokinetics of linezolid and co-amoxiclav in healthy human volunteers. J Antimicrob Chemother 50: 707-712 [Abstract] [Full Text]  
• Bronner, S., Pompei, D., Elkhaili, H., Dhoyen, N., Monteil, H., Jehl, F. (2001). Ex vivo 12 h bactericidal activity of oral co-amoxiclav (1.125 g) against {beta}-lactamase-producing Haemophilus influenzae. J Antimicrob Chemother 48: 501-506 [Abstract] [Full Text]