This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Medeiros, A A
Right arrow Articles by Jacoby, G A
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Medeiros, A A
Right arrow Articles by Jacoby, G A

 Previous Article  |  Next Article 

Antimicrob Agents Chemother. 1985 May; 27(5): 715-719

Five novel plasmid-determined beta-lactamases.

A A Medeiros, M Cohenford and G A Jacoby

ABSTRACT

Five novel plasmid-determined beta-lactamases named TLE-1, OXA-4, OXA-5, OXA-6, and OXA-7 were detected in ampicillin-resistant isolates of Escherichia coli and carbenicillin-resistant strains of Pseudomonas aeruginosa. TLE-1 resembled TEM-1 in substrate profile and reactions with inhibitors but differed in isoelectric point (5.55) and enzyme banding pattern on flat-bed electrofocusing.OXA-4, OXA-5, OXA-6, and OXA-7 hydrolyzed oxacillin, methicillin, and cloxacillin readily but differed from OXA-1, OXA-2, and OXA-3 in substrate profiles, inhibitor reactions, and isoelectric points (7.5 to 7.8).OXA-4 and OXA-6 were unusual for members of the OXA group in their sensitivity to inhibition by cloxacillin. OXA-5 and OXA-7 had isoelectric points close to that of SHV-1, emphasizing the need in beta-lactamase classification for studies in addition to isoelectric focusing. These five new enzymes bring the number of plasmid-determined beta-lactamases known in gram-negative organisms to more than 20. The evolution of such enzymatic diversity remains to be explored.

Antimicrob Agents Chemother. 1985 May; 27(5): 715-719




This article has been cited by other articles:

  • Jacoby, G. A. (2006). {beta}-Lactamase Nomenclature.. Antimicrob. Agents Chemother. 50: 1123-1129 [Full Text]  
  • Chmelnitsky, I., Carmeli, Y., Leavitt, A., Schwaber, M. J., Navon-Venezia, S. (2005). CTX-M-2 and a New CTX-M-39 Enzyme Are the Major Extended-Spectrum Beta-Lactamases in Multiple Escherichia coli Clones Isolated in Tel Aviv, Israel. Antimicrob. Agents Chemother. 49: 4745-4750 [Abstract] [Full Text]  
  • Alvarez, M., Tran, J. H., Chow, N., Jacoby, G. A. (2004). Epidemiology of Conjugative Plasmid-Mediated AmpC {beta}-Lactamases in the United States. Antimicrob. Agents Chemother. 48: 533-537 [Abstract] [Full Text]  
  • Pai, H., Jacoby, G. A. (2001). Sequences of the NPS-1 and TLE-1 {beta}-Lactamase Genes. Antimicrob. Agents Chemother. 45: 2947-2948 [Abstract] [Full Text]  
  • Leflon-Guibout, V., Speldooren, V., Heym, B., Nicolas-Chanoine, M.-H. (2000). Epidemiological Survey of Amoxicillin-Clavulanate Resistance and Corresponding Molecular Mechanisms in Escherichia coli Isolates in France: New Genetic Features of blaTEM Genes. Antimicrob. Agents Chemother. 44: 2709-2714 [Abstract] [Full Text]  
  • Ehrhardt, A. F., Sanders, C. C., Moland, E. S. (1999). Use of an Isogenic Escherichia coli Panel To Design Tests for Discrimination of beta -Lactamase Functional Groups of Enterobacteriaceae. Antimicrob. Agents Chemother. 43: 630-633 [Abstract] [Full Text]  
  • Marumo, K., Takeda, A., Nakamura, Y., Nakaya, K. (1999). Detection of OXA-4 {beta}-lactamase in Pseudomonas aeruginosa isolates by genetic methods. J Antimicrob Chemother 43: 187-193 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»