This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Martínez-Suárez, J V
Right arrow Articles by Pérez-Díaz, J C
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Martínez-Suárez, J V
Right arrow Articles by Pérez-Díaz, J C

 Previous Article  |  Next Article 

Antimicrob Agents Chemother. 1985 July; 28(1): 113-117

Transferable plasmid-linked chloramphenicol acetyltransferase conferring high-level resistance in Bacteroides uniformis.

J V Martínez-Suárez, F Baquero, M Reig and J C Pérez-Díaz

ABSTRACT

Bacteroides uniformis RYC3373 resistant to 64 micrograms of chloramphenicol per ml was isolated from a peritoneal pelvic abscess of a patient not previously treated with this drug. Chloramphenicol resistance was transferable at low frequency to a suitable Bacteroides fragilis recipient. The acquisition of resistance was linked to the presence of a 39.5-kilobase plasmid (pRYC3373), which was subsequently transferred to a secondary recipient. The transfer of Cm resistance occurred by a conjugation-like process. Donor and transconjugant strains produced chloramphenicol acetyltransferase constitutively. The Km for chloramphenicol was 40 microM, and its inactivation by 5-5'-dithiobis(2-nitrobenzoic acid) suggested its similarity to the type II enterobacterial enzymes encoded by different conjugative plasmids and also to a previously described enzyme of B. fragilis F47 and F48. The specific activity and the resistance level in pRYC3373-bearing strains were more than 10-fold higher than in the case of the enzyme from B. fragilis strains F47 and F48. The genetic basis of chloramphenicol acetyltransferase synthesis in Bacteroides spp. had not been previously established.

Antimicrob Agents Chemother. 1985 July; 28(1): 113-117




This article has been cited by other articles:

  • Wexler, H. M. (2007). Bacteroides: the Good, the Bad, and the Nitty-Gritty. Clin. Microbiol. Rev. 20: 593-621 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»