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Antimicrob Agents Chemother. 1985 July; 28(1): 154-156

A gene probe for TEM type beta-lactamases.

R C Cooksey, N C Clark and C Thornsberry

ABSTRACT

A restriction fragment of plasmid pBR322 bearing the TEM-1 beta-lactamase structural gene was electroeluted from agarose gels after digestion with EcoRI and HinfI. The 1-kilobase fragment was 32P-labeled and used to examine genetic relationships with nucleic acids encoding seven other beta-lactamase classes. The probe hybridized only with TEM-2 and OXA-2 class plasmids.

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Antimicrob Agents Chemother. 1985 July; 28(1): 154-156

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