This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Murray, B E Articles by Mederski-Samoraj, B Search for Related Content PubMed PubMed Citation Articles by Murray, B E Articles by Mederski-Samoraj, B

 Previous Article  |  Next Article 

Antimicrob Agents Chemother. 1986 December; 30(6): 861-864

Comparison of two beta-lactamase-producing strains of Streptococcus faecalis.

B E Murray, D A Church, A Wanger, K Zscheck, M E Levison, M J Ingerman, E Abrutyn and B Mederski-Samoraj

ABSTRACT

A second strain of enterococcus (PA) producing beta-lactamase (Bla+ phenotype) was compared with the previously reported Bla+ enterococcus, strain HH22. As with the original strain, there was a marked inoculum effect when PA was tested with penicillin, ampicillin, and piperacillin; no difference was noted with methicillin, cephalothin, imipenem, or vancomycin; the difference with ticarcillin was intermediate. High-level gentamicin resistance (Gmr) transferred from PA to an enterococcal recipient strain at a frequency approximately 100-fold lower than for HH22; all Gmr transconjugants from both strains were Bla+, but only PA showed linkage of Gmr and Bla+ with transfer of resistance to streptomycin, tetracycline, and chloramphenicol. EcoRI digestion of plasmid DNA from Gmr Bla+ transconjugants showed no similarities between the two strains. A 5.1-kilobase EcoRI Bla+-encoding fragment derived from HH22 was cloned into an Escherichia coli cloning vector and shown to hybridize to a 10.2-kilobase EcoRI fragment derived from PA; both fragments hybridized to an 840-base-pair staphylococcal Bla+ gene probe. These data indicate that the penicillinases are similar but encoded on different or differently arranged plasmids. The fact that both are transferable emphasizes the potential for this new streptococcal resistance determinant to disseminate.

---

Antimicrob Agents Chemother. 1986 December; 30(6): 861-864

This article has been cited by other articles:

• Nallapareddy, S. R., Wenxiang, H., Weinstock, G. M., Murray, B. E. (2005). Molecular Characterization of a Widespread, Pathogenic, and Antibiotic Resistance-Receptive Enterococcus faecalis Lineage and Dissemination of Its Putative Pathogenicity Island. J. Bacteriol. 187: 5709-5718 [Abstract] [Full Text]  
• Ono, S., Muratani, T., Matsumoto, T. (2005). Mechanisms of Resistance to Imipenem and Ampicillin in Enterococcus faecalis. Antimicrob. Agents Chemother. 49: 2954-2958 [Abstract] [Full Text]  
• Ida, T., Okamoto, R., Nonoyama, M., Irinoda, K., Kurazono, M., Inoue, M. (2002). Antagonism between Aminoglycosides and {beta}-Lactams in a Methicillin-Resistant Staphylococcus aureus Isolate Involves Induction of an Aminoglycoside-Modifying Enzyme. Antimicrob. Agents Chemother. 46: 1516-1521 [Abstract] [Full Text]  
• Chirurgi, V. A., Oster, S. E., Goldberg, A. A., McCabe, R. E. (1992). Nosocomial Acquisition of {beta}-Lactamase--Negative, Ampicillin-Resistant Enterococcus. Arch Intern Med 152: 1457-1461 [Abstract]  
• Wells, V. D., Wong, E. S., Murray, B. E., Coudron, P. E., Williams, D. S., Markowitz, S. M. (1992). Infections Due to Beta-Lactamase-producing, High-Level Gentamicin-resistant Enterococcus faecalis. ANN INTERN MED 116: 285-292 [Abstract]  
• Rupar, D. G., Fisher, M. C., Fletcher, H., Mortensen, J. (1989). Emergence of Isolates Resistant to Ampicillin. Arch Pediatr Adolesc Med 143: 1033-1037 [Abstract]  
• HOFFMANN, S. A., MOELLERING, R. C. Jr. (1987). The Enterococcus: "Putting the Bug in Our Ears". ANN INTERN MED 106: 757-761 [Abstract]