This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cooksey, R C
Right arrow Articles by Thornsberry, C
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cooksey, R C
Right arrow Articles by Thornsberry, C

 Previous Article  |  Next Article 

Antimicrob Agents Chemother. 1986 December; 30(6): 883-887

Microplate phosphocellulose binding assay for aminoglycoside-modifying enzymes.

R C Cooksey, B G Metchock and C Thornsberry

ABSTRACT

We modified the phosphocellulose binding assay for aminoglycoside-modifying enzymes (AMEs) by use of microdilution plates and a multichannel micropipette. Batteries of aminoglycoside substrates for screening organisms for the presence of AMEs as well as for subclassifying enzymes were prepared and stored in microdilution plates. When tested in parallel with the conventional tube reaction assay, the microplate assay yielded comparable radioactive counts and therefore equally correct identifications of AMEs in 32 isolates representing nine bacterial species. Other modifications, such as multichannel dispensing of crude enzyme preparations and radioisotopic precursors, provided a more rapid, convenient, and less expensive means of examining large collections of organisms for AMEs.

Antimicrob Agents Chemother. 1986 December; 30(6): 883-887




This article has been cited by other articles:

  • Clark, N. C., Olsvik, O., Swenson, J. M., Spiegel, C. A., Tenover, F. C. (1999). Detection of a Streptomycin/Spectinomycin Adenylyltransferase Gene (aadA) in Enterococcus faecalis. Antimicrob. Agents Chemother. 43: 157-160 [Abstract] [Full Text]  
  • Tsai, S. F., Zervos, M. J., Clewell, D. B., Donabedian, S. M., Sahm, D. F., Chow, J. W. (1998). A New High-Level Gentamicin Resistance Gene, aph(2")-Id, in Enterococcus spp.. Antimicrob. Agents Chemother. 42: 1229-1232 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»