This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Guay, G G Articles by Rothstein, D M Search for Related Content PubMed PubMed Citation Articles by Guay, G G Articles by Rothstein, D M

 Previous Article  |  Next Article 

Antimicrob Agents Chemother. 1993 February; 37(2): 191-198

Expression of the tetK gene from Staphylococcus aureus in Escherichia coli: comparison of substrate specificities of TetA(B), TetA(C), and TetK efflux proteins.

Department of Microbial Genetics, Lederle Laboratories, Pearl River, New York 10965.

ABSTRACT

The tetK gene, which encodes a tetracycline efflux pump from Staphylococcus aureus, was expressed in Escherichia coli by using an inducible, low-level expression system. The tetK gene, as well as the tetA(B) gene from the transposon Tn10 and the tetA(C) gene from plasmid pBR322, was subjected to the regulatory control of the lac repressor, and resistance to tetracycline was measured as a function of the isopropyl-beta-D-thiogalactopyranoside concentration. The maximum resistance of the E. coli strain containing the tetK construct was comparable to the maximum resistance of the strain containing the tetA(C) construct but was less than the resistance of the strain containing the tetA(B) construct. Overexpression of the tetK, tetA(B), or tetA(C) genes was toxic. When expression was regulated so that resistance to tetracycline was comparable, then the TetA(B) and TetA(C) proteins conferred very similar levels of resistance to a variety of tetracycline derivatives. In contrast, the TetK protein was less capable of conferring resistance to the tetracycline derivatives minocycline, 6-deoxy-6-demethyltetracycline, and doxycycline. The implications for the recognition of various tetracycline substituents by the TetK protein are discussed.

This article has been cited by other articles:

• Tuckman, M., Petersen, P. J., Howe, A. Y. M., Orlowski, M., Mullen, S., Chan, K., Bradford, P. A., Jones, C. H. (2007). Occurrence of Tetracycline Resistance Genes among Escherichia coli Isolates from the Phase 3 Clinical Trials for Tigecycline. Antimicrob. Agents Chemother. 51: 3205-3211 [Abstract] [Full Text]  
• Johanesen, P. A., Lyras, D., Bannam, T. L., Rood, J. I. (2001). Transcriptional Analysis of the tet(P) Operon from Clostridium perfringens. J. Bacteriol. 183: 7110-7119 [Abstract] [Full Text]  
• Ginn, S. L., Brown, M. H., Skurray, R. A. (2000). The TetA(K) Tetracycline/H+ Antiporter from Staphylococcus aureus: Mutagenesis and Functional Analysis of Motif C. J. Bacteriol. 182: 1492-1498 [Abstract] [Full Text]