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Antimicrob. Agents Chemother., Nov 1997, 2547-2549, Vol 41, No. 11
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Inhibitor-resistant TEM (IRT) beta-lactamases with different substitutions at position 244

L Bret, EB Chaibi, C Chanal-Claris, D Sirot, R Labia and J Sirot
Laboratoire de Bacteriologie, Faculte de Medecine, Clermont-Ferrand, France.

A novel inhibitor-resistant TEM (IRT) beta-lactamase was detected in an Escherichia coli isolate resistant to amoxicillin-clavulanate and susceptible to cephalothin. The substrate and inhibitor profiles of this beta-lactamase were similar to those of IRT-1 and IRT-2. The novel IRT's bla gene was sequenced, and the deduced amino acid sequence showed the amino acid replacement Arg for His-244 of the TEM-1 sequence. Substitutions for Arg-244 have been reported in three TEM-1 mutants: IRT-1 (which corresponds to TEM-31) (Cys), IRT-2/TEM-30 (Ser), and TEM-41 (Thr). We designated this novel beta-lactamase, which corresponds to TEM-51, IRT-15.

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