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Antimicrobial Agents and Chemotherapy, Jul 1997, 1601-1603, Vol 41, No. 7
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

Molecular analysis of katG gene mutations in strains of Mycobacterium tuberculosis complex from Africa

WH Haas, K Schilke, J Brand, B Amthor, K Weyer, PB Fourie, G Bretzel, V Sticht- Groh and HJ Bremer
Department of General Pediatrics, Children's Hospital, Heidelberg University, Germany. ic1@aixterm1.urz.uni-heidelberg.de

A sample of 124 isoniazid (INH)-resistant and 88 susceptible strains of Mycobacterium tuberculosis complex from south, central, and west Africa was analyzed by direct sequence analysis and PCR-restriction fragment length polymorphism analysis of their catalase-peroxidase (katG) genes. Point mutations at codon 315 were found in the genomes of 64% of INH- resistant strains, but no complete deletions were identified. Mutations at codon 463 were independent of INH resistance and were linked to the geographic origins of the strains.