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Antimicrobial Agents and Chemotherapy, January 1998, p. 190-193, Vol. 42, No. 1
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Cloning and Nucleotide Sequence of the DNA Gyrase gyrA Gene from Serratia marcescens and Characterization of Mutations in gyrA of Quinolone-Resistant Clinical Isolates

Jeong Hoon Kim,1 Eun Hee Cho,2 Kwang Seo Kim,3 Hak Yeop Kim,1 and Young Min Kim3,*

Department of Pharmacology and Molecular Biology, C&C Research Laboratories, Kyunggi-do 445-970,1 Department of Science Education, Chosun University, Kwangju 501-759,2 and Molecular Microbiology Laboratory, Department of Biology, Yonsei University, Seoul 120-749,3 Korea

Received 27 May 1997/Returned for modification 7 August 1997/Accepted 24 October 1997

The sequence of the DNA gyrase gyrA gene of Serratia marcescens ATCC 14756 was determined. An open reading frame of 2,640 nucleotides coding for a polypeptide with a calculated molecular mass of 97,460 was found, and its sequence complemented the sequence of an Escherichia coli gyrA temperature-sensitive mutation. Analysis of the PCR products of the quinolone resistance-determining regions of gyrA genes from six quinolone-resistant clinical isolates revealed a single amino acid substitution, Ser-83 to Arg or Asp-87 to Tyr, in all six mutants, suggesting that a mutational alteration in gyrA is a common mechanism of quinolone resistance in S. marcescens.

* Corresponding author. Mailing address: Molecular Microbiology Laboratory, Department of Biology, Yonsei University, Seoul 120-749, Korea. Phone: 82-2-361-2658. Fax: 82-2-312-5657. E-mail: young547{at}bubble.yonsei.ac.kr.

Antimicrobial Agents and Chemotherapy, January 1998, p. 190-193, Vol. 42, No. 1
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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