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Antimicrobial Agents and Chemotherapy, January 1998, p. 78-82, Vol. 42, No. 1
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Postantibiotic Effect in Escherichia coli Determined with Real-Time Metabolic Monitoring

Jeffrey M. Libby*

Molecular Devices Corporation, Sunnyvale, California 94089

Received 12 March 1997/Returned for modification 22 July 1997/Accepted 18 October 1997

Metabolic activity was used to quantify the delay in recovery of Escherichia coli after limited exposures to cefotaxime and piperacillin. This was measured with a microphysiometer, a device which measures changes in pH in the cellular environment, which in turn reflects the metabolic activity of the cells. The antibiotics were rapidly pumped into and flushed from each cellular environment. The length of time that the bacteria were exposed to either antibiotic was determined by programmed valve changes. Metabolic activity was measured during periods in which the instrument's pumps were off. Graphical analysis of the data was used to determine the postantibiotic effect. The lengths of the postantibiotic effects of both drugs (95 to 101 min) determined with the microphysiometer corresponded with the reappearance of short, highly motile cells in significant numbers.

* Mailing address: Molecular Devices Corporation, 1311 Orleans Dr., Sunnyvale, CA 94089. Phone: (408) 747-3507. Fax: (408) 747-3601. E-mail: jeff_libby{at}moldev.com.

Antimicrobial Agents and Chemotherapy, January 1998, p. 78-82, Vol. 42, No. 1
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Wiuff, C., Zappala, R. M., Regoes, R. R., Garner, K. N., Baquero, F., Levin, B. R. (2005). Phenotypic Tolerance: Antibiotic Enrichment of Noninherited Resistance in Bacterial Populations. Antimicrob. Agents Chemother. 49: 1483-1494 [Abstract] [Full Text]  


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