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Antimicrobial Agents and Chemotherapy, October 1998, p. 2690-2693, Vol. 42, No. 10
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Haemophilus ducreyi Is Susceptible to Protegrin

Kate Fortney,1 Patricia A. Totten,2 Robert I. Lehrer,3,4 and Stanley M. Spinola1,5,6,*

Departments of Medicine,1 Microbiology and Immunology,5 and Pathology and Laboratory Medicine,6 School of Medicine, Indiana University, Indianapolis, Indiana 46202; Department of Medicine, University of Washington, Seattle, Washington 981042; and Department of Medicine3 and Molecular Biology Institute,4 University of California, Los Angeles, Los Angeles, California 90095

Received 24 April 1998/Returned for modification 18 June 1998/Accepted 28 July 1998

Protegrins, potent antimicrobial peptides found in porcine leukocytes, have activity against the sexually transmitted pathogens Neisseria gonorrhoeae, Chlamydia trachomatis, and human immunodeficiency virus type 1. We tested synthetic protegrin 1 (PG-1) for activity against nine isolates of Haemophilus ducreyi, the etiologic agent of chancroid. The test organisms included CIP 542 (the type strain), 35000HP (a human-passaged variant of 35000), 35000HP-RSM2 (an isogenic D-glycero-D-manno-heptosyltransferase mutant of 35000HP), and six clinical isolates. The isolates were epidemiologically unrelated, represented three HindIII ribotypes, and had varying antimicrobial resistance patterns. In bactericidal assays, five isolates were rapidly killed by synthetic PG-1. In radial diffusion assays, all nine isolates were exquisitely sensitive to PG-1. These data highlight the potential of protegrins for development as topical agents to prevent many sexually transmitted diseases, including chancroid.


* Corresponding author. Mailing address: 435 Emerson Hall, 545 Barnhill Dr., Indiana University, Indianapolis, IN 46202-5124. Phone: (317) 274-8113. Fax: (317) 274-1587. E-mail: sspinola{at}iupui.edu.


Antimicrobial Agents and Chemotherapy, October 1998, p. 2690-2693, Vol. 42, No. 10
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.



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