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Antimicrobial Agents and Chemotherapy, October 1998, p. 2759-2761, Vol. 42, No. 10
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Characterization of IS18, an Element Capable of Activating the Silent aac(6')-Ij Gene of Acinetobacter sp. 13 Strain BM2716 by Transposition

Eric Rudant,1,2 Patrice Courvalin,1 and Thierry Lambert1,2,*

Unité des Agents Antibactériens, Institut Pasteur, 75724 Paris Cedex 15,1 and Centre d'Etudes Pharmaceutiques, 92296 Chatenay-Malabry,2 France

Received 11 March 1998/Returned for modification 27 April 1998/Accepted 16 July 1998

Insertion sequence IS18 was detected by analysis of the spontaneous aminoglycoside resistant mutant Acinetobacter sp. 13 strain BM2716-1. Insertion of the element upstream from the silent acetyltransferase gene aac(6')-Ij created a hybrid promoter that putatively accounts for the expression of the aminoglycoside resistance gene. The 1,074-bp IS18 element contained partially matched (20 out of 26 bases) terminal inverted repeats, one of which overlapped the 3' end of a 935-bp open reading frame potentially encoding a protein related to the transposases of the IS30 family. IS18 was found in 6 out of 29 strains of Acinetobacter sp. 13 but not in 10 strains each of A. baumannii and A. haemolyticus.

* Corresponding author. Mailing address: Unité des Agents Antibactériens, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France. Phone: (33) (1) 45 68 83 21. Fax: (33) (1) 45 68 83 19. E-mail: tlambert{at}pasteur.fr.

Antimicrobial Agents and Chemotherapy, October 1998, p. 2759-2761, Vol. 42, No. 10
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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