This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by González, I.
Right arrow Articles by de la Campa, A. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by González, I.
Right arrow Articles by de la Campa, A. G.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, November 1998, p. 2792-2798, Vol. 42, No. 11
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Fluoroquinolone Resistance Mutations in the parC, parE, and gyrA Genes of Clinical Isolates of Viridans Group Streptococci

Irene González,1 Marios Georgiou,1,2 Fernando Alcaide,3 Delia Balas,1 Josefina Liñares,3 and Adela G. de la Campa1,*

Unidad de Genética Bacteriana (Consejo Superior de Investigaciones Científicas), Centro Nacional de Biología Fundamental, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid,1 and Servicio de Microbiología, Hospital Princeps d'Espanya, Ciutat Sanitària i Universitaria de Belltvitge, 08907 L'Hospitalet de Llobregat, Barcelona,3 Spain, and University of Greenwich, London, United Kingdom2

Received 15 April 1998/Returned for modification 13 July 1998/Accepted 8 August 1998

The nucleotide sequences of the quinolone resistance-determining regions (QRDRs) of the parC and gyrA genes from seven ciprofloxacin-resistant (Cpr) isolates of viridans group streptococci (two high-level Cpr Streptococcus oralis and five low-level Cpr Streptococcus mitis isolates) were determined and compared with those obtained from susceptible isolates. The nucleotide sequences of the QRDRs of the parE and gyrB genes from the five low-level Cpr S. mitis isolates and from the NCTC 12261 type strain were also analyzed. Four of these low-level Cpr isolates had changes affecting the subunits of DNA topoisomerase IV: three in Ser-79 (to Phe or Ile) of ParC and one in ParE at a position not previously described to be involved in quinolone resistance (Pro-424). One isolate did not show any mutation. The two high-level Cpr S. oralis isolates showed mutations affecting equivalent residue positions of ParC and GyrA, namely, Ser-79 to Phe and Ser-81 to Phe or Tyr, respectively. The parC mutations were able to transform Streptococcus pneumoniae to ciprofloxacin resistance, while the gyrA mutations transformed S. pneumoniae only when mutations in parC were present. These results suggest that DNA topoisomerase IV is a primary target of ciprofloxacin in viridans group streptococci, DNA gyrase being a secondary target.

* Corresponding author. Mailing address: Unidad de Genética Bacteriana (Consejo Superior de Investigaciones Científicas), Centro Nacional de Biología Fundamental, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain. Phone: (34) 91-509-7904. Fax: (34) 91-509-7918. E-mail: agcampa{at}isciii.es.

Antimicrobial Agents and Chemotherapy, November 1998, p. 2792-2798, Vol. 42, No. 11
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Wu, H.-M., Janapatla, R. P., Ho, Y.-R., Hung, K.-H., Wu, C.-W., Yan, J.-J., Wu, J.-J. (2008). Emergence of Fluoroquinolone Resistance in Group B Streptococcal Isolates in Taiwan. Antimicrob. Agents Chemother. 52: 1888-1890 [Abstract] [Full Text]  
  • Balsalobre, L., de la Campa, A. G. (2008). Fitness of Streptococcus pneumoniae Fluoroquinolone-Resistant Strains with Topoisomerase IV Recombinant Genes. Antimicrob. Agents Chemother. 52: 822-830 [Abstract] [Full Text]  
  • Ip, M., Chau, S. S. L., Chi, F., Tang, J., Chan, P. K. (2007). Fluoroquinolone Resistance in Atypical Pneumococci and Oral Streptococci: Evidence of Horizontal Gene Transfer of Fluoroquinolone Resistance Determinants from Streptococcus pneumoniae. Antimicrob. Agents Chemother. 51: 2690-2700 [Abstract] [Full Text]  
  • Nakano, K., Lapirattanakul, J., Nomura, R., Nemoto, H., Alaluusua, S., Gronroos, L., Vaara, M., Hamada, S., Ooshima, T., Nakagawa, I. (2007). Streptococcus mutans Clonal Variation Revealed by Multilocus Sequence Typing. J. Clin. Microbiol. 45: 2616-2625 [Abstract] [Full Text]  
  • Delorme, C., Poyart, C., Ehrlich, S. D., Renault, P. (2007). Extent of Horizontal Gene Transfer in Evolution of Streptococci of the Salivarius Group. J. Bacteriol. 189: 1330-1341 [Abstract] [Full Text]  
  • Escudero, J. A., San Millan, A., Catalan, A., de la Campa, A. G., Rivero, E., Lopez, G., Dominguez, L., Moreno, M. A., Gonzalez-Zorn, B. (2007). First Characterization of Fluoroquinolone Resistance in Streptococcus suis. Antimicrob. Agents Chemother. 51: 777-782 [Abstract] [Full Text]  
  • Ferrandiz, M. J., Perez-Trallero, E., Marimon, J. M., de la Campa, A. G. (2005). Clinical Isolates of the Spain14-5 Clone of Streptococcus pneumoniae Carry a Recombinant rpoB Gene. Antimicrob. Agents Chemother. 49: 4811-4813 [Full Text]  
  • Ling, J. M., Chan, E. W., Lam, A. W., Cheng, A. F. (2003). Mutations in Topoisomerase Genes of Fluoroquinolone-Resistant Salmonellae in Hong Kong. Antimicrob. Agents Chemother. 47: 3567-3573 [Abstract] [Full Text]  
  • Martin-Galiano, A. J., Balsalobre, L., Fenoll, A., de la Campa, A. G. (2003). Genetic Characterization of Optochin-Susceptible Viridans Group Streptococci. Antimicrob. Agents Chemother. 47: 3187-3194 [Abstract] [Full Text]  
  • Seppala, H., Haanpera, M., Al-Juhaish, M., Jarvinen, H., Jalava, J., Huovinen, P. (2003). Antimicrobial susceptibility patterns and macrolide resistance genes of viridans group streptococci from normal flora. J Antimicrob Chemother 52: 636-644 [Abstract] [Full Text]  
  • Balsalobre, L., Ferrandiz, M. J., Linares, J., Tubau, F., de la Campa, A. G. (2003). Viridans Group Streptococci Are Donors in Horizontal Transfer of Topoisomerase IV Genes to Streptococcus pneumoniae. Antimicrob. Agents Chemother. 47: 2072-2081 [Abstract] [Full Text]  
  • Martin-Galiano, A. J., de la Campa, A. G. (2003). High-Efficiency Generation of Antibiotic-Resistant Strains of Streptococcus pneumoniae by PCR and Transformation. Antimicrob. Agents Chemother. 47: 1257-1261 [Abstract] [Full Text]  
  • de la Campa, A. G., Ferrandiz, M.-J., Tubau, F., Pallares, R., Manresa, F., Linares, J. (2003). Genetic Characterization of Fluoroquinolone-Resistant Streptococcus pneumoniae Strains Isolated during Ciprofloxacin Therapy from a Patient with Bronchiectasis. Antimicrob. Agents Chemother. 47: 1419-1422 [Abstract] [Full Text]  
  • Martin-Galiano, A. J., Gorgojo, B., Kunin, C. M., de la Campa, A. G. (2002). Mefloquine and New Related Compounds Target the F0 Complex of the F0F1 H+-ATPase of Streptococcus pneumoniae. Antimicrob. Agents Chemother. 46: 1680-1687 [Abstract] [Full Text]  
  • Bast, D. J., de Azavedo, J. C. S., Tam, T. Y., Kilburn, L., Duncan, C., Mandell, L. A., Davidson, R. J., Low, D. E. (2001). Interspecies Recombination Contributes Minimally to Fluoroquinolone Resistance in Streptococcus pneumoniae. Antimicrob. Agents Chemother. 45: 2631-2634 [Abstract] [Full Text]  
  • Thornsberry, C., Karlowsky, J. A., Sahm, D. F., Jorgensen, J. H., Weigel, L. M., Swenson, J. M., Whitney, C. G., Tenover, F. C., Ferraro, M. J. (2001). Levofloxacin-Resistant Streptococcus pneumoniae: Second Look. Antimicrob. Agents Chemother. 45: 2183-2184 [Full Text]  
  • Hakenbeck, R., Balmelle, N., Weber, B., Gardes, C., Keck, W., de Saizieu, A. (2001). Mosaic Genes and Mosaic Chromosomes: Intra- and Interspecies Genomic Variation of Streptococcus pneumoniae. Infect. Immun. 69: 2477-2486 [Abstract] [Full Text]  
  • Wilson, D. L., Abner, S. R., Newman, T. C., Mansfield, L. S., Linz, J. E. (2000). Identification of Ciprofloxacin-Resistant Campylobacter jejuni by Use of a Fluorogenic PCR Assay. J. Clin. Microbiol. 38: 3971-3978 [Abstract] [Full Text]  
  • Guerin, F., Varon, E., Hoï, A. B., Gutmann, L., Podglajen, I. (2000). Fluoroquinolone Resistance Associated with Target Mutations and Active Efflux in Oropharyngeal Colonizing Isolates of Viridans Group Streptococci. Antimicrob. Agents Chemother. 44: 2197-2200 [Abstract] [Full Text]  
  • Ferrándiz, M. J., Fenoll, A., Liñares, J., De La Campa, A. G. (2000). Horizontal Transfer of parC and gyrA in Fluoroquinolone-Resistant Clinical Isolates of Streptococcus pneumoniae. Antimicrob. Agents Chemother. 44: 840-847 [Abstract] [Full Text]  
  • Ferrándiz, M. J., Oteo, J., Aracil, B., Gómez-Garcés, J. L., De La Campa, A. G. (1999). Drug Efflux and parC Mutations Are Involved in Fluoroquinolone Resistance in Viridans Group Streptococci. Antimicrob. Agents Chemother. 43: 2520-2523 [Abstract] [Full Text]  
  • de Azavedo, J. C. S., Trpeski, L., Pong-Porter, S., Matsumura, S., The Canadian Bacterial Surveillance Network, , Low, D. E. (1999). In Vitro Activities of Fluoroquinolones against Antibiotic-Resistant Blood Culture Isolates of Viridans Group Streptococci from across Canada. Antimicrob. Agents Chemother. 43: 2299-2301 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»