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Antimicrobial Agents and Chemotherapy, December 1998, p. 3113-3116, Vol. 42, No. 12
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Novel OXA-10-Derived Extended-Spectrum beta -Lactamases Selected In Vivo or In Vitro

P. Mugnier,1,dagger I. Casin,1,2 A. T. Bouthors,1 and E. Collatz1,*

Laboratoire de Recherche Moléculaire sur les Antibiotiques, UFR Broussais-Hôtel Dieu and UFR Pitié-Salpêtrière, Université Paris VI,1 and Laboratoire de Bactériologie, Hôpital Saint-Louis, Université Paris VII,2 Paris, France

Received 25 February 1998/Returned for modification 3 June 1998/Accepted 20 September 1998

A clinical isolate of Pseudomonas aeruginosa, PAe191, was found to be highly resistant to all anti-Pseudomonas beta -lactam antibiotics (except imipenem) and resistant also to aminoglycosides. It produced a beta -lactamase (with an apparent pI of 7.6) which was not inhibited by clavulanic acid. Cloning and characterization of the beta -lactamase gene showed that it coded for a novel extended-spectrum OXA-10 variant, called OXA-19, which differed from OXA-10 by nine amino acids and from OXA-13 by two, i.e., Asn in position 73 (Asn73) instead of Ser and Asp157 instead of Gly. Asparagine in position 157 is implicated in resistance to ceftazidime, while the amino acid in position 73, in this variant, seems to condition the level of resistance to penicillins. The oxa19 gene was found to be inserted, in a typical integron structure, immediately downstream from an aac(6')-Ib gene coding for an aminoglycoside acetyltransferase variant, which was called AAC(6')-Ib9.

* Corresponding author. Mailing address: L.R.M.A., Université Paris VI, 15, rue de l'Ecole de Médecine, 75270 Paris Cedex 06, France. Phone: 33-1-42 34 68.65. Fax: 33-1-43.25.68.12. E-mail: collatz{at}ccr.jussieu.fr.

dagger Present address: Department of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, United Kingdom.

Antimicrobial Agents and Chemotherapy, December 1998, p. 3113-3116, Vol. 42, No. 12
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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