This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bayer, A. S.
Right arrow Articles by Fowler, V. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bayer, A. S.
Right arrow Articles by Fowler, V. G., Jr.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, December 1998, p. 3169-3172, Vol. 42, No. 12
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

In Vitro Resistance to Thrombin-Induced Platelet Microbicidal Protein among Clinical Bacteremic Isolates of Staphylococcus aureus Correlates with an Endovascular Infectious Source

Arnold S. Bayer,1,2,* Darwin Cheng,1 Michael R. Yeaman,1,2 G. Ralph Corey,3 R. Scott McClelland,3 Lizzie J. Harrel,4 and Vance G. Fowler Jr.3

The St. John's Cardiovascular Research Center and the Division of Infectious Diseases, Harbor-UCLA Medical Center, Torrance, California 905091; the UCLA School of Medicine, Los Angeles, California 900242; and the Departments of Internal Medicine3 and Microbiology,4 Duke University Medical Center, Durham, North Carolina 27705

Received 1 June 1998/Returned for modification 2 September 1998/Accepted 16 September 1998

Platelet microbicidal proteins (PMPs), small cationic peptides released at sites of endovascular damage, kill common bloodstream pathogens in vitro. Our group previously showed that in vitro resistance of clinical staphylococcal and viridans group streptococcal bacteremic strains to PMPs correlated with the diagnosis of infective endocarditis (IE) (Wu et al., Antimicrob. Agents Chemother. 38:729-732, 1994). However, that study was limited by (i) the small number of Staphylococcus aureus isolates from IE patients, (ii) the retrospective nature of the case definitions, and (iii) the diverse geographic sources of strains. The present study evaluated the in vitro PMP susceptibility phenotype of a large number of staphylococcemic isolates (n = 60), collected at a single medical center and categorized by defined and validated clinical criteria. A significantly higher proportion of staphylococcemic strains from patients with IE was PMP resistant in vitro than the proportion of strains from patients with soft tissue sepsis (83% and 33%, respectively; P < 0.01). Moreover, the levels of PMP resistance (mean percent survival of strains after 2-h exposure to PMP in vitro) were significantly higher for isolates from patients with IE and with vascular catheter sepsis than for strains from patients with abscess sepsis (P < 0.005 and P < 0.01, respectively). These data further support the concept that bloodstream pathogens that exhibit innate or acquired PMP resistance have a survival advantage with respect to either the induction or progression of endovascular infections.

* Corresponding author. Mailing address: Division of Infectious Diseases, Harbor-UCLA Medical Center, Bldg RB2/Room 225, 1000 West Carson St., Torrance, CA 90509. Phone: (310) 222-6422. Fax: (310) 782-2016. E-mail: Bayer{at}HUMC.EDU.

Antimicrobial Agents and Chemotherapy, December 1998, p. 3169-3172, Vol. 42, No. 12
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Mukhopadhyay, K., Whitmire, W., Xiong, Y. Q., Molden, J., Jones, T., Peschel, A., Staubitz, P., Adler-Moore, J., McNamara, P. J., Proctor, R. A., Yeaman, M. R., Bayer, A. S. (2007). In vitro susceptibility of Staphylococcus aureus to thrombin-induced platelet microbicidal protein-1 (tPMP-1) is influenced by cell membrane phospholipid composition and asymmetry. Microbiology 153: 1187-1197 [Abstract] [Full Text]  
  • Bayer, A. S., Kupferwasser, L. I., Brown, M. H., Skurray, R. A., Grkovic, S., Jones, T., Mukhopadhay, K., Yeaman, M. R. (2006). Low-Level Resistance of Staphylococcus aureus to Thrombin-Induced Platelet Microbicidal Protein 1 In Vitro Associated with qacA Gene Carriage Is Independent of Multidrug Efflux Pump Activity.. Antimicrob. Agents Chemother. 50: 2448-2454 [Abstract] [Full Text]  
  • Dankert, J., van der Werff, J., Joldersma, W., Zaat, S. A. J. (2006). Interleukin 1{alpha} Increases the Susceptibility of Rabbits to Experimental Viridans Streptococcal Endocarditis. Infect. Immun. 74: 947-952 [Abstract] [Full Text]  
  • Weidenmaier, C., Peschel, A., Kempf, V. A. J., Lucindo, N., Yeaman, M. R., Bayer, A. S. (2005). DltABCD- and MprF-Mediated Cell Envelope Modifications of Staphylococcus aureus Confer Resistance to Platelet Microbicidal Proteins and Contribute to Virulence in a Rabbit Endocarditis Model. Infect. Immun. 73: 8033-8038 [Abstract] [Full Text]  
  • Sakoulas, G., Eliopoulos, G. M., Fowler, V. G. Jr., Moellering, R. C. Jr., Novick, R. P., Lucindo, N., Yeaman, M. R., Bayer, A. S. (2005). Reduced Susceptibility of Staphylococcus aureus to Vancomycin and Platelet Microbicidal Protein Correlates with Defective Autolysis and Loss of Accessory Gene Regulator (agr) Function. Antimicrob. Agents Chemother. 49: 2687-2692 [Abstract] [Full Text]  
  • Zhang, H., Morikawa, K., Ohta, T., Kato, Y. (2005). In vitro resistance to the CS{alpha}{beta}-type antimicrobial peptide ASABF- is conferred by overexpression of sigma factor sigB in Staphylococcus aureus. J Antimicrob Chemother 55: 686-691 [Abstract] [Full Text]  
  • Bell, G., Gouyon, P.-H. (2003). Arming the enemy: the evolution of resistance to self-proteins. Microbiology 149: 1367-1375 [Abstract] [Full Text]  
  • Yeaman, M. R., Yount, N. Y. (2003). Mechanisms of Antimicrobial Peptide Action and Resistance. Pharmacol. Rev. 55: 27-55 [Abstract] [Full Text]  
  • Jacqueline, C., Batard, E., Perez, L., Boutoille, D., Hamel, A., Caillon, J., Kergueris, M.-F., Potel, G., Bugnon, D. (2002). In Vivo Efficacy of Continuous Infusion versus Intermittent Dosing of Linezolid Compared to Vancomycin in a Methicillin-Resistant Staphylococcus aureus Rabbit Endocarditis Model. Antimicrob. Agents Chemother. 46: 3706-3711 [Abstract] [Full Text]  
  • Kupferwasser, L. I., Yeaman, M. R., Shapiro, S. M., Nast, C. C., Bayer, A. S. (2002). In Vitro Susceptibility to Thrombin-Induced Platelet Microbicidal Protein Is Associated With Reduced Disease Progression and Complication Rates in Experimental Staphylococcus aureus Endocarditis: Microbiological, Histopathologic, and Echocardiographic Analyses. Circulation 105: 746-752 [Abstract] [Full Text]  
  • Dhawan, V. K., Bayer, A. S., Yeaman, M. R. (2000). Thrombin-Induced Platelet Microbicidal Protein Susceptibility Phenotype Influences the Outcome of Oxacillin Prophylaxis and Therapy of Experimental Staphylococcus aureus Endocarditis. Antimicrob. Agents Chemother. 44: 3206-3209 [Abstract] [Full Text]  
  • Mercier, R.-C., Rybak, M. J., Bayer, A. S., Yeaman, M. R. (2000). Influence of Platelets and Platelet Microbicidal Protein Susceptibility on the Fate of Staphylococcus aureus in an In Vitro Model of Infective Endocarditis. Infect. Immun. 68: 4699-4705 [Abstract] [Full Text]  
  • Bayer, A. S., Prasad, R., Chandra, J., Koul, A., Smriti, M., Varma, A., Skurray, R. A., Firth, N., Brown, M. H., Koo, S.-P., Yeaman, M. R. (2000). In Vitro Resistance of Staphylococcus aureus to Thrombin-Induced Platelet Microbicidal Protein Is Associated with Alterations in Cytoplasmic Membrane Fluidity. Infect. Immun. 68: 3548-3553 [Abstract] [Full Text]  
  • Gopal, A. K., Fowler, V. G. Jr, Shah, M., Gesty-Palmer, D., Marr, K. A., McClelland, R. S., Kong, L. K., Gottlieb, G. S., Lanclos, K., Li, J., Sexton, D. J., Corey, G. R. (2000). Prospective Analysis of Staphylococcus aureus Bacteremia in Nonneutropenic Adults With Malignancy. JCO 18: 1110-1110 [Abstract] [Full Text]  
  • Kupferwasser, L. I., Skurray, R. A., Brown, M. H., Firth, N., Yeaman, M. R., Bayer, A. S. (1999). Plasmid-Mediated Resistance to Thrombin-Induced Platelet Microbicidal Protein in Staphylococci: Role of the qacA Locus. Antimicrob. Agents Chemother. 43: 2395-2399 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»