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Antimicrobial Agents and Chemotherapy, February 1998, p. 394-398, Vol. 42, No. 2
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Intrinsic Resistance to Inhibitors of Fatty Acid
Biosynthesis in Pseudomonas aeruginosa Is Due to Efflux:
Application of a Novel Technique for Generation of Unmarked
Chromosomal Mutations for the Study of Efflux Systems
Herbert P.
Schweizer*
Department of Microbiology, Colorado State
University, Fort Collins, Colorado 80523
Received 2 June 1997/Returned for modification 7 August
1997/Accepted 11 November 1997
Many strains of Pseudomonas aeruginosa are resistant to
the antibiotics cerulenin and thiolactomycin, potent inhibitors of bacterial fatty acid biosynthesis. A novel yeast Flp recombinase-based technique was used to isolate an unmarked mexAB-oprM
deletion encoding an efflux system mediating resistance to multiple
antibiotics in P. aeruginosa. The experiments showed that
the MexAB-OprM system is responsible for the intrinsic resistance of
this bacterium to cerulenin and thiolactomycin. Whereas thiolactomycin
was not a substrate of the MexCD-OprJ pump expressed in a
(mexAB-oprM) nfxB mutant, cerulenin was
efficiently effluxed by the MexCD-OprJ system. It was also found that
the MexAB-OprM system is capable of efflux of irgasan, a broad-spectrum
antimicrobial compound used in media selective for Pseudomonas.
*
Mailing address: Department of Microbiology, Colorado
State University, Fort Collins, CO 80523-1677. Phone: (970) 491-3536. Fax: (970) 491-1815. E-mail:
hschweizer{at}vines.colostate.edu.
Antimicrobial Agents and Chemotherapy, February 1998, p. 394-398, Vol. 42, No. 2
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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