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Antimicrobial Agents and Chemotherapy, February 1998, p. 436-439, Vol. 42, No. 2
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Nucleotide and Amino Acid Sequences of the Metallo-beta -Lactamase, ImiS, from Aeromonas veronii bv. sobria

T. R. Walsh,1,* W. A. Neville,2 M. H. Haran,3 D. Tolson,2 D. J. Payne,3 J. H. Bateson,2 A. P. Macgowan,4 and P. M. Bennett1

Bristol Centre for Antimicrobial Research and Evaluation, Department of Microbiology and Pathology, Medical School, University of Bristol, Bristol BS8 1TD,1 Southmead Health Services, NHS Trust, Bristol BS10 5NB,4 and Analytical Sciences and Med Chem, SmithKline Beecham Pharmaceuticals, New Froniters, Harlow, Essex,2 United Kingdom, and Microbiology Research, SmithKline Beecham Pharmaceuticals, Collegeville, Pennsylvania 194263

Received 21 April 1997/Returned for modification 27 August 1997/Accepted 25 November 1997

The Aeromonas veronii bv. sobria metallo-beta -lactamase gene, imiS, was cloned. The imiS open reading frame extends for 762 bp and encodes a protein of 254 amino acids with a secreted modified protein of 227 amino acids and a predicted pI of 8.1. To confirm the predicted sequence, purified ImiS was digested and the resulting peptides were identified, yielding an identical sequence for ImiS, with 98% identity to CphA. Both possessed the putative active-site sequence Asn-Tyr-His-Thr-Asp at positions 88 to 92, which is unique to the Aeromonas metallo-beta -lactamases.

* Corresponding author. Mailing address: Bristol Centre for Antimicrobial Research and Evaluation, Department of Microbiology and Pathology, School of Medical Sciences, University Walk, University of Bristol, Bristol BS8 1TD, United Kingdom. Phone: 0117 9282567. Fax: 0117 9287896. E-mail: T.R.WALSH{at}BRISTOL.AC.UK.

Antimicrobial Agents and Chemotherapy, February 1998, p. 436-439, Vol. 42, No. 2
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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