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Antimicrobial Agents and Chemotherapy, April 1998, p. 974-977, Vol. 42, No. 4
Division of Biomedical Sciences, University
of California, Riverside, California 92521
Received 6 October 1997/Returned for modification 19 December
1997/Accepted 6 February 1998
We demonstrated that a 2% suspension of Pseudomonas
aeruginosa alginate completely blocked the diffusion of
gentamicin and tobramycin, but not that of carbenicillin, illustrating
how alginate production can help protect P. aeruginosa
growing within alginate microcolonies in patients with cystic fibrosis
(CF) from the effects of aminoglycosides. This aminoglycoside diffusion
barrier was degraded with a semipurified preparation of P. aeruginosa alginate lyase, suggesting that this enzyme deserves
consideration as an adjunctive agent for CF patients colonized by
mucoid strains of P. aeruginosa.
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Alginate Lyase Promotes Diffusion of
Aminoglycosides through the Extracellular Polysaccharide of Mucoid
Pseudomonas aeruginosa
*
Corresponding author. Mailing address: Division of
Biomedical Sciences, University of California, Riverside, CA 92521. Phone: (909) 787-4569. Fax: (909) 787-5504. E-mail:
neal.schiller{at}ucr.edu.
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