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Antimicrobial Agents and Chemotherapy, April 1998, p. 974-977, Vol. 42, No. 4
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Alginate Lyase Promotes Diffusion of Aminoglycosides through the Extracellular Polysaccharide of Mucoid Pseudomonas aeruginosa

Richard A. Hatch and Neal L. Schiller*

Division of Biomedical Sciences, University of California, Riverside, California 92521

Received 6 October 1997/Returned for modification 19 December 1997/Accepted 6 February 1998

We demonstrated that a 2% suspension of Pseudomonas aeruginosa alginate completely blocked the diffusion of gentamicin and tobramycin, but not that of carbenicillin, illustrating how alginate production can help protect P. aeruginosa growing within alginate microcolonies in patients with cystic fibrosis (CF) from the effects of aminoglycosides. This aminoglycoside diffusion barrier was degraded with a semipurified preparation of P. aeruginosa alginate lyase, suggesting that this enzyme deserves consideration as an adjunctive agent for CF patients colonized by mucoid strains of P. aeruginosa.

* Corresponding author. Mailing address: Division of Biomedical Sciences, University of California, Riverside, CA 92521. Phone: (909) 787-4569. Fax: (909) 787-5504. E-mail: neal.schiller{at}ucr.edu.

Antimicrobial Agents and Chemotherapy, April 1998, p. 974-977, Vol. 42, No. 4
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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