Previous Article | Next Article 
Antimicrobial Agents and Chemotherapy, May 1998, p. 1229-1232, Vol. 42, No. 5
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
A New High-Level Gentamicin Resistance Gene,
aph(2")-Id, in Enterococcus spp.
Shane F.
Tsai,1
Marcus J.
Zervos,2
Don B.
Clewell,3
Susan M.
Donabedian,2
Daniel F.
Sahm,4,
and
Joseph W.
Chow1,5,*
Research and Medical Service, Department of
Veterans' Affairs Medical Center,1 and
Wayne State University School of
Medicine,5 Detroit, Michigan 48201;
William Beaumont Hospital, Royal Oak, Michigan
480732;
Departments of Biologic and
Materials Sciences and Microbiology and Immunology, University of
Michigan, Ann Arbor, Michigan 481093; and
Department of Pathology, Jewish Hospital at Washington
University Medical Center, St. Louis, Missouri 631104
Received 5 August 1997/Returned for modification 3 December
1997/Accepted 18 February 1998
Enterococcus casseliflavus UC73 is a clinical blood
isolate with high-level resistance to gentamicin. DNA
preparations from UC73 failed to hybridize with intragenic probes
for aac(6')-Ie-aph(2")-Ia and aph(2")-Ic. A
4-kb fragment from UC73 was cloned and found to confer resistance to
gentamicin in Escherichia coli DH5
transformants. Nucleotide sequence analysis revealed the presence
of a 906-bp open reading frame whose deduced amino acid sequence had a
region with homology to the aminoglycoside-modifying enzyme APH(2")-Ic and to the C-terminal domain of the bifunctional enzyme
AAC(6')-APH(2"). The gene is designated aph(2")-Id, and its
observed phosphotransferase activity is designated APH(2")-Id. A
PCR-generated intragenic probe hybridized to the genomic DNA from 17 of
118 enterococcal clinical isolates (108 with high-level gentamicin
resistance) from five hospitals. All 17 were vancomycin-resistant
Enterococcus faecium isolates, and pulsed-field typing
revealed three distinct clones. The combination of
ampicillin plus either amikacin or neomycin exhibited synergistic
killing against E. casseliflavus UC73. Screening and
interpretation of high-level aminoglycoside resistance in enterococci
may need to be modified to include detection of APH(2")-Id.
*
Corresponding author. Mailing address: Division of
Infectious Diseases, Wayne State University School of Medicine, 4160 John R, Suite 2140, Detroit, MI 48201-2021. Phone: (313) 745-9649. Fax:
(313) 763-9905. E-mail: waichung{at}umich.edu.
Present address: MRL Pharmaceutical Services, Inc., Reston, VA
20190.
Antimicrobial Agents and Chemotherapy, May 1998, p. 1229-1232, Vol. 42, No. 5
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
This article has been cited by other articles:
-
Young, P. G., Walanj, R., Lakshmi, V., Byrnes, L. J., Metcalf, P., Baker, E. N., Vakulenko, S. B., Smith, C. A.
(2009). The Crystal Structures of Substrate and Nucleotide Complexes of Enterococcus faecium Aminoglycoside-2''-Phosphotransferase-IIa [APH(2'')-IIa] Provide Insights into Substrate Selectivity in the APH(2'') Subfamily. J. Bacteriol.
191: 4133-4143
[Abstract]
[Full Text]
-
Toth, M., Chow, J. W., Mobashery, S., Vakulenko, S. B.
(2009). Source of Phosphate in the Enzymic Reaction as a Point of Distinction among Aminoglycoside 2''-Phosphotransferases. J. Biol. Chem.
284: 6690-6696
[Abstract]
[Full Text]
-
Chow, V. C. Y., Hawkey, P. M., Chan, E. W. C., Chin, M. L., Au, T. K., Fung, D. K. C., Chan, R. C. Y.
(2007). High-Level Gentamicin Resistance Mediated by a Tn4001-Like Transposon in Seven Nonclonal Hospital Isolates of Streptococcus pasteurianus. Antimicrob. Agents Chemother.
51: 2508-2513
[Abstract]
[Full Text]
-
Aslangul, E., Massias, L., Meulemans, A., Chau, F., Andremont, A., Courvalin, P., Fantin, B., Ruimy, R.
(2006). Acquired Gentamicin Resistance by Permeability Impairment in Enterococcus faecalis. Antimicrob. Agents Chemother.
50: 3615-3621
[Abstract]
[Full Text]
-
Chen, Y.-G., Qu, T.-T., Yu, Y.-S., Zhou, J.-Y., Li, L.-J.
(2006). Insertion sequence ISEcp1-like element connected with a novel aph(2'') allele [aph(2'')-Ie] conferring high-level gentamicin resistance and a novel streptomycin adenylyltransferase gene in Enterococcus.. J Med Microbiol
55: 1521-1525
[Abstract]
[Full Text]
-
Nallapareddy, S. R., Singh, K. V., Murray, B. E.
(2006). Construction of Improved Temperature-Sensitive and Mobilizable Vectors and Their Use for Constructing Mutations in the Adhesin-Encoding acm Gene of Poorly Transformable Clinical Enterococcus faecium Strains. Appl. Environ. Microbiol.
72: 334-345
[Abstract]
[Full Text]
-
Aslangul, E., Ruimy, R., Chau, F., Garry, L., Andremont, A., Fantin, B.
(2005). Relationship between the Level of Acquired Resistance to Gentamicin and Synergism with Amoxicillin in Enterococcus faecalis. Antimicrob. Agents Chemother.
49: 4144-4148
[Abstract]
[Full Text]
-
Perreten, V., Vorlet-Fawer, L., Slickers, P., Ehricht, R., Kuhnert, P., Frey, J.
(2005). Microarray-Based Detection of 90 Antibiotic Resistance Genes of Gram-Positive Bacteria. J. Clin. Microbiol.
43: 2291-2302
[Abstract]
[Full Text]
-
Lopes, M. d. F. S., Ribeiro, T., Martins, M. P., Tenreiro, R., Crespo, M. T. B.
(2003). Gentamicin resistance in dairy and clinical enterococcal isolates and in reference strains. J Antimicrob Chemother
52: 214-219
[Abstract]
[Full Text]
-
Vakulenko, S. B., Mobashery, S.
(2003). Versatility of Aminoglycosides and Prospects for Their Future. Clin. Microbiol. Rev.
16: 430-450
[Abstract]
[Full Text]
-
Murdoch, D. R., Mirrett, S., Harrell, L. J., Donabedian, S. M., Zervos, M. J., Reller, L. B.
(2003). Comparison of Microscan Broth Microdilution, Synergy Quad Plate Agar Dilution, and Disk Diffusion Screening Methods for Detection of High-Level Aminoglycoside Resistance in Enterococcus Species. J. Clin. Microbiol.
41: 2703-2705
[Abstract]
[Full Text]
-
Vakulenko, S. B., Donabedian, S. M., Voskresenskiy, A. M., Zervos, M. J., Lerner, S. A., Chow, J. W.
(2003). Multiplex PCR for Detection of Aminoglycoside Resistance Genes in Enterococci. Antimicrob. Agents Chemother.
47: 1423-1426
[Abstract]
[Full Text]
-
Donabedian, S. M., Thal, L. A., Hershberger, E., Perri, M. B., Chow, J. W., Bartlett, P., Jones, R., Joyce, K., Rossiter, S., Gay, K., Johnson, J., Mackinson, C., Debess, E., Madden, J., Angulo, F., Zervos, M. J.
(2003). Molecular Characterization of Gentamicin-Resistant Enterococci in the United States: Evidence of Spread from Animals to Humans through Food. J. Clin. Microbiol.
41: 1109-1113
[Abstract]
[Full Text]
-
Lee, H. K., Vakulenko, S. B., Clewell, D. B., Lerner, S. A., Chow, J. W.
(2002). Mutations in the aph(2")-Ic Gene Are Responsible for Increased Levels of Aminoglycoside Resistance. Antimicrob. Agents Chemother.
46: 3253-3256
[Abstract]
[Full Text]
-
Vergis, E. N., Hayden, M. K., Chow, J. W., Snydman, D. R., Zervos, M. J., Linden, P. K., Wagener, M. M., Schmitt, B., Muder, R. R.
(2001). Determinants of Vancomycin Resistance and Mortality Rates in Enterococcal Bacteremia: A Prospective Multicenter Study. ANN INTERN MED
135: 484-492
[Abstract]
[Full Text]
-
Tenorio, C., Zarazaga, M., Martinez, C., Torres, C.
(2001). Bifunctional Enzyme 6'-N-Aminoglycoside Acetyltransferase-2"-O- Aminoglycoside Phosphotransferase in Lactobacillus and Pediococcus Isolates of Animal Origin. J. Clin. Microbiol.
39: 824-825
[Full Text]
-
Kao, S. J., You, I., Clewell, D. B., Donabedian, S. M., Zervos, M. J., Petrin, J., Shaw, K. J., Chow, J. W.
(2000). Detection of the High-Level Aminoglycoside Resistance Gene aph(2")-Ib in Enterococcus faecium. Antimicrob. Agents Chemother.
44: 2876-2879
[Abstract]
[Full Text]
-
Kak, V., Donabedian, S. M., Zervos, M. J., Kariyama, R., Kumon, H., Chow, J. W.
(2000). Efficacy of Ampicillin plus Arbekacin in Experimental Rabbit Endocarditis Caused by an Enterococcus faecalis Strain with High-Level Gentamicin Resistance. Antimicrob. Agents Chemother.
44: 2545-2546
[Abstract]
[Full Text]
-
Murray, B. E.
(2000). Vancomycin-Resistant Enterococcal Infections. NEJM
342: 710-721
[Full Text]
-
Papaparaskevas, J., Vatopoulos, A., Tassios, P. T., Avlami, A., Legakis, N. J., Kalapothaki, V.
(2000). Diversity among high-level aminoglycoside-resistant enterococci. J Antimicrob Chemother
45: 277-283
[Abstract]
[Full Text]
-
Weinbren, M. J., Johnson, A. P., Woodford, N.
(2000). Defining high-level gentamicin resistance in enterococci. J Antimicrob Chemother
45: 404-405
[Full Text]
-
van den Braak, N., van Belkum, A., Kreft, D., te Witt, R., Verbrugh, H. A., Endtz, H. Ph.
(1999). The prevalence and clonal expansion of high-level gentamicin-resistant enterococci isolated from blood cultures in a Dutch university hospital. J Antimicrob Chemother
44: 795-798
[Abstract]
[Full Text]
-
Ostrowsky, B., Eliopoulos, G. M.
(1999). Analytic Reviews : Enterococcal Infections: Resistance Mechanisms, Treatment Strategies, and Hospital Issues: Ostrowsky B, Eliopoulos, GM Enterococcal Infections Resistance Mechanisms, Treatment Strategies, and Hospital Issues J Intensive Care Med 1999,14.62-73. J Intensive Care Med
14: 62-73