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Antimicrobial Agents and Chemotherapy, May 1998, p. 1249-1252, Vol. 42, No. 5
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Characterization of grlA, grlB, gyrA, and gyrB Mutations in 116 Unrelated Isolates of Staphylococcus aureus and Effects of Mutations on Ciprofloxacin MIC

Franz-Josef Schmitz,1,2,* Mark E. Jones,2 Basia Hofmann,1 Birgit Hansen,1 Sibylle Scheuring,3 Marc Lückefahr,1 Ad Fluit,2 Jan Verhoef,2 Ulrich Hadding,1 Hans-Peter Heinz,1 and Karl Köhrer3

Institute for Medical Microbiology and Virology1 and Molekularbiologisches Zentrallabor im Biologisch-Medizinischen Forschungszentrum,3 Heinrich-Heine University Düsseldorf, Düsseldorf, Germany, and Eijkman-Winkler Institute for Medical Microbiology, Utrecht University, Utrecht, The Netherlands2

Received 25 August 1997/Returned for modification 18 December 1997/Accepted 25 February 1998

One hundred sixteen unrelated clinical isolates of Staphylococcus aureus (70 ciprofloxacin resistant and 46 ciprofloxacin susceptible) from eight countries were studied for the presence of mutations in the grlA, grlB, gyrA, and gyrB gene loci. Two mutations within grlA (located at codons 80 and 84) and two mutations within gyrA (located at codons 84 and 88) were clearly associated with ciprofloxacin resistance, although other mutations detected within the four genes studied may also contribute to decreased susceptibility.

* Corresponding author. Mailing address: Institute for Medical Microbiology and Virology, Heinrich-Heine-Universität Düsseldorf, Universitätsstrabeta e 1, Geb. 22.21, 40225 Düsseldorf, Germany. Phone: 49-2132-72040. Fax: 49-211-811-5323.

Antimicrobial Agents and Chemotherapy, May 1998, p. 1249-1252, Vol. 42, No. 5
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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