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Antimicrobial Agents and Chemotherapy, June 1998, p. 1428-1432, Vol. 42, No. 6
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Use of a Continuous-Culture Biofilm System To Study the Antimicrobial Susceptibilities of Gardnerella vaginalis and Lactobacillus acidophilus

F. Muli and J. K. Struthers*

Department of Medical Microbiology, Manchester Royal Infirmary, and the University of Manchester, Manchester M13 9WL, United Kingdom

Received 2 December 1997/Returned for modification 5 February 1998/Accepted 26 March 1998

Gardnerella vaginalis and Lactobacillus acidophilus have been shown to grow to high titers in a simple biofilm system. This system was used in the present investigation to compare the biofilm-eradicating concentrations (BECs) of amoxicillin, clindamycin, erythromycin, and metronidazole to standard tube MIC and minimum bactericidal concentration (MBC) results. With the lactobacillus, the BEC/tube MBC ratio was at least 16:1, while for G. vaginalis the ratio varied from 2:1 to 512:1. The simple continuous-culture system used in the present investigation is ideal for investigating the BEC for bacteria involved in complex ecological situations such as bacterial vaginosis and may be useful for the identification of the most effective and selective antibiotic therapy.

* Corresponding author. Present mailing address: Public Health Laboratory, Coventry and Warwickshire Hospital, Stoney Stanton Road, Coventry CV1 4FH, United Kingdom. Phone: 44 (1203) 844121. Fax: 44 (1203) 220081. E-mail: covphl{at}globalnet.co.uk.

Antimicrobial Agents and Chemotherapy, June 1998, p. 1428-1432, Vol. 42, No. 6
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Budhani, R. K., Struthers, J. K. (1998). Interaction of Streptococcus pneumoniae and Moraxella catarrhalis: Investigation of the Indirect Pathogenic Role of beta -Lactamase-Producing Moraxellae by Use of a Continuous-Culture Biofilm System. Antimicrob. Agents Chemother. 42: 2521-2526 [Abstract] [Full Text]  


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