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Antimicrobial Agents and Chemotherapy, July 1998, p. 1689-1694, Vol. 42, No. 7
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Gene Amplification in Leishmania tarentolae Selected for Resistance to Sodium Stibogluconate

Anass Haimeur and Marc Ouellette*

Centre de Recherche en Infectiologie du CHUL and Département de Biologie Médicale, Division de Microbiologie, Université Laval, Québec, Canada

Received 17 February 1998/Returned for modification 17 March 1998/Accepted 5 May 1998

Leishmania tarentolae promastigotes were selected step by step for resistance to sodium stibogluconate (Pentostam). Mutants resistant to antimony-containing drugs and cross-resistant to arsenite were therefore obtained. Amplification of one common locus was observed in several independent sodium stibogluconate-resistant mutants, and the locus amplified was novel. The copy number of the amplified locus was related to the level of resistance to pentavalent antimony. The gene responsible for antimony resistance was isolated by transfection and was shown to correspond to an open reading frame coding for 770 amino acids. The putative gene product did not exhibit significant homology with sequences present in data banks, and the putative role of this protein in antimony resistance is discussed.

* Corresponding author. Mailing address: Centre de Recherche en Infectiologie, 2705, boul. Laurier, Sainte-Foy, Québec G1V 4G2, Canada. Phone: (418) 654-2705. Fax: (418) 654-2715. E-mail: Marc.Ouellette{at}crchul.ulaval.ca.

Antimicrobial Agents and Chemotherapy, July 1998, p. 1689-1694, Vol. 42, No. 7
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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