This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Haimeur, A.
Right arrow Articles by Ouellette, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Haimeur, A.
Right arrow Articles by Ouellette, M.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, July 1998, p. 1689-1694, Vol. 42, No. 7
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Gene Amplification in Leishmania tarentolae Selected for Resistance to Sodium Stibogluconate

Anass Haimeur and Marc Ouellette*

Centre de Recherche en Infectiologie du CHUL and Département de Biologie Médicale, Division de Microbiologie, Université Laval, Québec, Canada

Received 17 February 1998/Returned for modification 17 March 1998/Accepted 5 May 1998

Leishmania tarentolae promastigotes were selected step by step for resistance to sodium stibogluconate (Pentostam). Mutants resistant to antimony-containing drugs and cross-resistant to arsenite were therefore obtained. Amplification of one common locus was observed in several independent sodium stibogluconate-resistant mutants, and the locus amplified was novel. The copy number of the amplified locus was related to the level of resistance to pentavalent antimony. The gene responsible for antimony resistance was isolated by transfection and was shown to correspond to an open reading frame coding for 770 amino acids. The putative gene product did not exhibit significant homology with sequences present in data banks, and the putative role of this protein in antimony resistance is discussed.


* Corresponding author. Mailing address: Centre de Recherche en Infectiologie, 2705, boul. Laurier, Sainte-Foy, Québec G1V 4G2, Canada. Phone: (418) 654-2705. Fax: (418) 654-2715. E-mail: Marc.Ouellette{at}crchul.ulaval.ca.


Antimicrobial Agents and Chemotherapy, July 1998, p. 1689-1694, Vol. 42, No. 7
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:

  • Wyllie, S., Vickers, T. J., Fairlamb, A. H. (2008). Roles of Trypanothione S-Transferase and Tryparedoxin Peroxidase in Resistance to Antimonials. Antimicrob. Agents Chemother. 52: 1359-1365 [Abstract] [Full Text]  
  • MITTAL, M. K., RAI, S., ASHUTOSH, , RAVINDER, , GUPTA, S., SUNDAR, S., GOYAL, N. (2007). CHARACTERIZATION OF NATURAL ANTIMONY RESISTANCE IN LEISHMANIA DONOVANI ISOLATES. Am J Trop Med Hyg 76: 681-688 [Abstract] [Full Text]  
  • Ashutosh, , Sundar, S., Goyal, N. (2007). Molecular mechanisms of antimony resistance in Leishmania. J Med Microbiol 56: 143-153 [Abstract] [Full Text]  
  • Gourbal, B., Sonuc, N., Bhattacharjee, H., Legare, D., Sundar, S., Ouellette, M., Rosen, B. P., Mukhopadhyay, R. (2004). Drug Uptake and Modulation of Drug Resistance in Leishmania by an Aquaglyceroporin. J. Biol. Chem. 279: 31010-31017 [Abstract] [Full Text]  
  • Guimond, C., Trudel, N., Brochu, C., Marquis, N., Fadili, A. E., Peytavi, R., Briand, G., Richard, D., Messier, N., Papadopoulou, B., Corbeil, J., Bergeron, M. G., Legare, D., Ouellette, M. (2003). Modulation of gene expression in Leishmania drug resistant mutants as determined by targeted DNA microarrays. Nucleic Acids Res 31: 5886-5896 [Abstract] [Full Text]  
  • Brochu, C., Wang, J., Roy, G., Messier, N., Wang, X.-Y., Saravia, N. G., Ouellette, M. (2003). Antimony Uptake Systems in the Protozoan Parasite Leishmania and Accumulation Differences in Antimony-Resistant Parasites. Antimicrob. Agents Chemother. 47: 3073-3079 [Abstract] [Full Text]  
  • Carter, K. C., Sundar, S., Spickett, C., Pereira, O. C., Mullen, A. B. (2003). The In Vivo Susceptibility of Leishmania donovani to Sodium Stibogluconate Is Drug Specific and Can Be Reversed by Inhibiting Glutathione Biosynthesis. Antimicrob. Agents Chemother. 47: 1529-1535 [Abstract] [Full Text]  
  • Carter, K. C., Mullen, A. B., Sundar, S., Kenney, R. T. (2001). Efficacies of Vesicular and Free Sodium Stibogluconate Formulations against Clinical Isolates of Leishmania donovani. Antimicrob. Agents Chemother. 45: 3555-3559 [Abstract] [Full Text]  
  • Wiesgigl, M., Clos, J. (2001). Heat Shock Protein 90 Homeostasis Controls Stage Differentiation in Leishmania donovani. Mol. Biol. Cell 12: 3307-3316 [Abstract] [Full Text]  
  • Ephros, M., Bitnun, A., Shaked, P., Waldman, E., Zilberstein, D. (1999). Stage-Specific Activity of Pentavalent Antimony against Leishmania donovani Axenic Amastigotes. Antimicrob. Agents Chemother. 43: 278-282 [Abstract] [Full Text]  
  • Shaked-Mishan, P., Ulrich, N., Ephros, M., Zilberstein, D. (2001). Novel Intracellular SbV Reducing Activity Correlates with Antimony Susceptibility in Leishmania donovani. J. Biol. Chem. 276: 3971-3976 [Abstract] [Full Text]