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Antimicrobial Agents and Chemotherapy, August 1998, p. 1895-1899, Vol. 42, No. 8
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Effect of Rebamipide, a Novel Antiulcer Agent, on Helicobacter pylori Adhesion to Gastric Epithelial Cells

Shunji Hayashi,1,* Toshiro Sugiyama,2 Ken-Ichi Amano,3 Hiroshi Isogai,4 Emiko Isogai,5 Miki Aihara,6 Mikio Kikuchi,6 Masahiro Asaka,2 Kenji Yokota,7 Keiji Oguma,7 Nobuhiro Fujii,8 and Yoshikazu Hirai1

Department of Microbiology, Jichi Medical School, Tochigi-ken 329-0498,1 Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo 060-8638,2 Central Research Laboratory, Akita University School of Medicine, Akita 010-8543,3 Animal Experimentation Center4 and Department of Microbiology,8 Sapporo Medical University School of Medicine, Sapporo 060-8556, Department of Hygiene, Health Sciences University of Hokkaido, Hokkaido 061-0293,5 Otsuka Pharmaceutical Co., Ltd., Tokushima 771-0192,6 and Department of Bacteriology, Okayama University Medical School, Okayama 700-8558,7 Japan

Received 20 October 1997/Returned for modification 6 February 1998/Accepted 8 May 1998

Helicobacter pylori is a major etiological agent in gastroduodenal disorders. The adhesion of H. pylori to human gastric epithelial cells is the initial step of H. pylori infection. Inhibition of H. pylori adhesion is thus a therapeutic target in the prevention of H. pylori infection. Experiments were performed to evaluate the effect of rebamipide, a novel antiulcer agent, on H. pylori adhesion to gastric epithelial cells. MKN-28 and MKN-45 cells, derived from human gastric carcinomas, were used as target cells. Ten H. pylori strains isolated from patients with chronic gastritis and gastric ulcer were used in the study. We evaluated the effect of rebamipide on H. pylori adhesion to MKN-28 and MKN-45 cells quantitatively using our previously established enzyme-linked immunosorbent assay. The adhesion of H. pylori to MKN-28 and MKN-45 cells was significantly inhibited by pretreatment of these cells with 100 µg of rebamipide per ml. However, the adhesion was not affected by the pretreatment of H. pylori with rebamipide. On the other hand, the viabilities of H. pylori, MKN-28 cells, and MKN-45 cells were not affected by rebamipide. Our studies suggest that rebamipide inhibits the adhesion of H. pylori to gastric epithelial cells.

* Corresponding author. Mailing address: Department of Microbiology, Jichi Medical School, 3311-1 Yakushiji, Minamikawachi-machi, Tochigi-ken 329-0498, Japan. Phone: 81-285-58-7332. Fax: 81-285-44-1175. E-mail: shunhaya{at}jichi.ac.jp.

Antimicrobial Agents and Chemotherapy, August 1998, p. 1895-1899, Vol. 42, No. 8
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Copyright © 1998 by the American Society for Microbiology. All rights reserved.