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Antimicrobial Agents and Chemotherapy, August 1998, p. 1966-1972, Vol. 42, No. 8
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Structure of CARB-4 and AER-1 CarbenicillinHydrolyzing beta -Lactamases

François Sanschagrin, Noureddine Bejaoui, and Roger C. Levesque*

Microbiologie Moléculaire et Génie des Protéines, Sciences de la Vie et de la Santé, Faculté de Médecine et Pavillon Charles-Eugène Marchand, Université Laval, Ste-Foy, Québec, Canada G1K 7P4

Received 23 May 1997/Returned for modification 21 September 1997/Accepted 30 May 1998

We determined the nucleotide sequences of blaCARB-4 encoding CARB-4 and deduced a polypeptide of 288 amino acids. The gene was characterized as a variant of group 2c carbenicillin-hydrolyzing beta -lactamases such as PSE-4, PSE-1, and CARB-3. The level of DNA homology between the bla genes for these beta -lactamases varied from 98.7 to 99.9%, while that between these genes and blaCARB-4 encoding CARB-4 was 86.3%. The blaCARB-4 gene was acquired from some other source because it has a G+C content of 39.1%, compared to a G+C content of 67% for typical Pseudomonas aeruginosa genes. DNA sequencing revealed that blaAER-1 shared 60.8% DNA identity with blaPSE-3 encoding PSE-3. The deduced AER-1 beta -lactamase peptide was compared to class A, B, C, and D enzymes and had 57.6% identity with PSE-3, including an STHK tetrad at the active site. For CARB-4 and AER-1, conserved canonical amino acid boxes typical of class A beta -lactamases were identified in a multiple alignment. Analysis of the DNA sequences flanking blaCARB-4 and blaAER-1 confirmed the importance of gene cassettes acquired via integrons in bla gene distribution.

* Corresponding author. Mailing address: Microbiologie Moléculaire et Génie des Protéines, Sciences de la Vie et de la Santé, Faculté de Médecine et Pavillon Charles-Eugène Marchand, Université Laval, Ste-Foy, Québec, Canada G1K 7P4. Phone: (418) 656-3070. Fax: (418) 656-7176. E-mail: rclevesq{at}rsvs.ulaval.ca.

Antimicrobial Agents and Chemotherapy, August 1998, p. 1966-1972, Vol. 42, No. 8
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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