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Antimicrobial Agents and Chemotherapy, August 1998, p. 2024-2031, Vol. 42, No. 8
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Cloning and Nucleotide Sequences of the Topoisomerase IV parC and parE Genes of Mycoplasma hominis

Cécile M. Bébéar,1,2 Alain Charron,1 Joseph Marie Bové,2 Christiane Bébéar,1 and Joel Renaudin2,*

Laboratoire de Bactériologie, Université Bordeaux 2, 33076 Bordeaux Cedex,1 and Laboratoire de Biologie Cellulaire et Moléculaire, Institut National de la Recherche Agronomique, 33883 Villenave d'Ornon Cedex,2 France

Received 26 January 1998/Returned for modification 12 May 1998/Accepted 8 June 1998

The topoisomerase IV parC and parE genes from the wall-less organism Mycoplasma hominis PG21 were cloned and sequenced. The coupled genes are located far from the DNA gyrase genes gyrA and gyrB. They encode proteins of 639 and 866 amino acids, respectively. As expected, the encoded ParE and ParC proteins exhibit higher homologies with the topoisomerase IV subunits of the gram-positive bacteria Staphylococcus aureus and Streptococcus pneumoniae than with their Escherichia coli counterparts. The conserved regions include the Tyr residue of the active site and the region involved in quinolone resistance (quinolone resistance-determining region [QRDR]) in ParC and the ATP-binding site and the QRDR in ParE.

* Corresponding author. Mailing address: Laboratoire de Biologie Cellulaire et Moléculaire, Institut National de la Recherche Agronomique, Domaine de la Grande Ferrade, BP 81, 33883 Villenave d'Ornon Cedex, France. Phone: (33) 5 56 84 31 51. Fax: (33) 5 56 84 31 59. E-mail: renaudin{at}bordeaux.inra.fr.

Antimicrobial Agents and Chemotherapy, August 1998, p. 2024-2031, Vol. 42, No. 8
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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