This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Naas, T.
Right arrow Articles by Nordmann, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Naas, T.
Right arrow Articles by Nordmann, P.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, August 1998, p. 2074-2083, Vol. 42, No. 8
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Molecular Characterization of OXA-20, a Novel Class D beta -Lactamase, and Its Integron from Pseudomonas aeruginosa

Thierry Naas,1,* Wladimir Sougakoff,2 Anne Casetta,3 and Patrice Nordmann1,3

Service de Bactériologie-Virologie, Hôpital Antoine Béclère, Faculté de Médecine Paris-Sud, 92141 Clamart Cedex,1 Service de Bactériologie-Hygiène, Faculté de Médecine Pitié-Salpêtrière, 75634 Paris Cedex 13,2 and Service de Bactériologie-Virologie, Hôpital de Bicêtre, Faculté de Médecine Paris-Sud, 94274 Le Kremlin-Bicêtre,3 France

Received 3 December 1997/Returned for modification 3 March 1998/Accepted 10 June 1998

The Pseudomonas aeruginosa Mus clinical isolate produces OXA-18, a pI 5.5 class D extended-spectrum beta -lactamase totally inhibited by clavulanic acid (L. N. Philippon, T. Naas, A.-T. Bouthors, V. Barakett, and P. Nordmann, Antimicrob. Agents Chemother. 41:2188-2195, 1997). A second beta -lactamase was cloned, and the recombinant Escherichia coli clone pPL10 expressed a pI 7.4 beta -lactamase which conferred high levels of amoxicillin and ticarcillin resistance and which was partially inhibited by clavulanic acid. The 2.5-kb insert from pPL10 was sequenced, and a 266-amino-acid protein (OXA-20) was deduced; this protein has low amino acid identity with most of the class D beta -lactamases except OXA-2, OXA-15, and OXA-3 (75% amino acid identity with each). OXA-20 is a restricted-spectrum oxacillinase and is unusually inhibited by clavulanic acid. OXA-20 is a peculiar beta -lactamase because its translation initiates with a TTG (leucine) codon, which is rarely used as a translational origin in bacteria. Exploration of the genetic environment of oxa20 revealed the presence of the following integron features: (i) a second antibiotic resistance gene, aacA4; (ii) an intI1 gene; and (iii) two 59-base elements, each associated with either oxa20 or aacA4. This integron is peculiar because it lacks the 3' conserved region, and therefore is not a sul1-associated integron like most of them, and because its 3' end is located within tnpR, a gene involved in the transposition of Tn5393, a gram-negative transposon. P. aeruginosa Mus produces two novel and unrelated oxacillinases, OXA-18 and OXA-20, both of which are inhibited by clavulanic acid.

* Corresponding author. Mailing address: Service de Bactériologie-Virologie, Hôpital Antoine Béclère, 157 rue de-la-Porte de-Trivaux, 92141 Clamart Cedex, France. Phone: 33-1-45-37-42-98. Fax: 33-1-46-32-67-96. E-mail: Thierry.Naas{at}kb.u-psud.fr.

Antimicrobial Agents and Chemotherapy, August 1998, p. 2074-2083, Vol. 42, No. 8
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Naas, T., Namdari, F., Bogaerts, P., Huang, T.-D., Glupczynski, Y., Nordmann, P. (2008). Genetic Structure Associated with blaOXA-18, Encoding a Clavulanic Acid-Inhibited Extended-Spectrum Oxacillinase. Antimicrob. Agents Chemother. 52: 3898-3904 [Abstract] [Full Text]  
  • Brown, S., Amyes, S. (2006). OXA {beta}-lactamases in Acinetobacter: the story so far. J Antimicrob Chemother 57: 1-3 [Abstract] [Full Text]  
  • Heritier, C., Poirel, L., Fournier, P.-E., Claverie, J.-M., Raoult, D., Nordmann, P. (2005). Characterization of the Naturally Occurring Oxacillinase of Acinetobacter baumannii. Antimicrob. Agents Chemother. 49: 4174-4179 [Abstract] [Full Text]  
  • Mantengoli, E., Rossolini, G. M. (2005). Tn5393d, a Complex Tn5393 Derivative Carrying the PER-1 Extended-Spectrum {beta}-Lactamase Gene and Other Resistance Determinants. Antimicrob. Agents Chemother. 49: 3289-3296 [Abstract] [Full Text]  
  • Poirel, L., Van De Loo, M., Mammeri, H., Nordmann, P. (2005). Association of Plasmid-Mediated Quinolone Resistance with Extended-Spectrum {beta}-Lactamase VEB-1. Antimicrob. Agents Chemother. 49: 3091-3094 [Abstract] [Full Text]  
  • Giuliani, F., Docquier, J.-D., Riccio, M. L., Pagani, L., Rossolini, G. M. (2005). OXA-46, a New Class D {beta}-Lactamase of Narrow Substrate Specificity Encoded by a blaVIM-1-Containing Integron from a Pseudomonas aeruginosa Clinical Isolate. Antimicrob. Agents Chemother. 49: 1973-1980 [Abstract] [Full Text]  
  • Girlich, D., Naas, T., Nordmann, P. (2004). OXA-60, a Chromosomal, Inducible, and Imipenem-Hydrolyzing Class D {beta}-Lactamase from Ralstonia pickettii. Antimicrob. Agents Chemother. 48: 4217-4225 [Abstract] [Full Text]  
  • Aubert, D., Girlich, D., Naas, T., Nagarajan, S., Nordmann, P. (2004). Functional and Structural Characterization of the Genetic Environment of an Extended-Spectrum {beta}-Lactamase blaVEB Gene from a Pseudomonas aeruginosa Isolate Obtained in India. Antimicrob. Agents Chemother. 48: 3284-3290 [Abstract] [Full Text]  
  • Zarrilli, R., Crispino, M., Bagattini, M., Barretta, E., Di Popolo, A., Triassi, M., Villari, P. (2004). Molecular Epidemiology of Sequential Outbreaks of Acinetobacter baumannii in an Intensive Care Unit Shows the Emergence of Carbapenem Resistance. J. Clin. Microbiol. 42: 946-953 [Abstract] [Full Text]  
  • Colombo, M.-L., Hanique, S., Baurin, S. L., Bauvois, C., De Vriendt, K., Van Beeumen, J. J., Frere, J.-M., Joris, B. (2004). The ybxI Gene of Bacillus subtilis 168 Encodes a Class D {beta}-Lactamase of Low Activity. Antimicrob. Agents Chemother. 48: 484-490 [Abstract] [Full Text]  
  • Aubert, D., Naas, T., Nordmann, P. (2003). IS1999 Increases Expression of the Extended-Spectrum {beta}-Lactamase VEB-1 in Pseudomonas aeruginosa. J. Bacteriol. 185: 5314-5319 [Abstract] [Full Text]  
  • Vakulenko, S. B., Mobashery, S. (2003). Versatility of Aminoglycosides and Prospects for Their Future. Clin. Microbiol. Rev. 16: 430-450 [Abstract] [Full Text]  
  • Casin, I., Hanau-Bercot, B., Podglajen, I., Vahaboglu, H., Collatz, E. (2003). Salmonella enterica Serovar Typhimurium blaPER-1-Carrying Plasmid pSTI1 Encodes an Extended-Spectrum Aminoglycoside 6'-N-Acetyltransferase of Type Ib. Antimicrob. Agents Chemother. 47: 697-703 [Abstract] [Full Text]  
  • Naas, T., Bellais, S., Nordmann, P. (2003). Molecular and biochemical characterization of a carbapenem-hydrolysing {beta}-lactamase from Flavobacterium johnsoniae. J Antimicrob Chemother 51: 267-273 [Abstract] [Full Text]  
  • Gombac, F., Riccio, M. L., Rossolini, G. M., Lagatolla, C., Tonin, E., Monti-Bragadin, C., Lavenia, A., Dolzani, L. (2002). Molecular Characterization of Integrons in Epidemiologically Unrelated Clinical Isolates of Acinetobacter baumannii from Italian Hospitals Reveals a Limited Diversity of Gene Cassette Arrays. Antimicrob. Agents Chemother. 46: 3665-3668 [Abstract] [Full Text]  
  • Bert, F., Branger, C., Lambert-Zechovsky, N. (2002). Identification of PSE and OXA {beta}-lactamase genes in Pseudomonas aeruginosa using PCR-restriction fragment length polymorphism. J Antimicrob Chemother 50: 11-18 [Abstract] [Full Text]  
  • Franceschini, N., Boschi, L., Pollini, S., Herman, R., Perilli, M., Galleni, M., Frere, J.-M., Amicosante, G., Rossolini, G. M. (2001). Characterization of OXA-29 from Legionella (Fluoribacter) gormanii: Molecular Class D beta -Lactamase with Unusual Properties. Antimicrob. Agents Chemother. 45: 3509-3516 [Abstract] [Full Text]  
  • Bradford, P. A. (2001). Extended-Spectrum {beta}-Lactamases in the 21st Century: Characterization, Epidemiology, and Detection of This Important Resistance Threat. Clin. Microbiol. Rev. 14: 933-951 [Abstract] [Full Text]  
  • Petrella, S., Clermont, D., Casin, I., Jarlier, V., Sougakoff, W. (2001). Novel Class A {beta}-Lactamase Sed-1 from Citrobacter sedlakii: Genetic Diversity of {beta}-Lactamases within the Citrobacter Genus. Antimicrob. Agents Chemother. 45: 2287-2298 [Abstract] [Full Text]  
  • Maguire, A. J., Brown, D. F. J., Gray, J. J., Desselberger, U. (2001). Rapid Screening Technique for Class 1 Integrons in Enterobacteriaceae and Nonfermenting Gram-Negative Bacteria and Its Use in Molecular Epidemiology. Antimicrob. Agents Chemother. 45: 1022-1029 [Abstract] [Full Text]  
  • Poirel, L., Girlich, D., Naas, T., Nordmann, P. (2001). OXA-28, an Extended-Spectrum Variant of OXA-10 {beta}-Lactamase from Pseudomonas aeruginosa and Its Plasmid- and Integron-Located Gene. Antimicrob. Agents Chemother. 45: 447-453 [Abstract] [Full Text]  
  • L'Abée-Lund, T. M., Sørum, H. (2000). Functional Tn5393-Like Transposon in the R Plasmid pRAS2 from the Fish Pathogen Aeromonas salmonicida subspecies salmonicida Isolated in Norway. Appl. Environ. Microbiol. 66: 5533-5535 [Abstract] [Full Text]  
  • Sundin, G. W. (2000). Examination of base pair variants of the strA-strB streptomycin resistance genes from bacterial pathogens of humans, animals and plants. J Antimicrob Chemother 46: 848-849 [Full Text]  
  • Ploy, M.-C., Denis, F., Courvalin, P., Lambert, T. (2000). Molecular Characterization of Integrons in Acinetobacter baumannii: Description of a Hybrid Class 2 Integron. Antimicrob. Agents Chemother. 44: 2684-2688 [Abstract] [Full Text]  
  • Nordmann, P., Poirel, L., Kubina, M., Casetta, A., Naas, T. (2000). Biochemical-Genetic Characterization and Distribution of OXA-22, a Chromosomal and Inducible Class D beta -Lactamase from Ralstonia (Pseudomonas) pickettii. Antimicrob. Agents Chemother. 44: 2201-2204 [Abstract] [Full Text]  
  • Donald, H. M., Scaife, W., Amyes, S. G. B., Young, H.-K. (2000). Sequence Analysis of ARI-1, a Novel OXA beta -Lactamase, Responsible for Imipenem Resistance in Acinetobacter baumannii 6B92. Antimicrob. Agents Chemother. 44: 196-199 [Abstract] [Full Text]  
  • Rosser, S. J., Young, H.-K. (1999). Identification and characterization of class 1 integrons in bacteria from an aquatic environment. J Antimicrob Chemother 44: 11-18 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»