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Antimicrobial Agents and Chemotherapy, August 1998, p. 2146-2149, Vol. 42, No. 8
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Iron-Limited Biofilms of Candida albicans and Their Susceptibility to Amphotericin B

George S. Baillie and L. Julia Douglas*

Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, United Kingdom

Received 2 March 1998/Returned for modification 18 May 1998/Accepted 9 June 1998

Biofilms of Candida albicans were grown in vitro under iron limitation and at a low growth rate to simulate conditions for implant-associated biofilms in vivo. Their properties were compared with those of glucose-limited biofilms grown under analogous conditions. At steady state, the adherent cell populations of iron-limited biofilms were double those of glucose-limited biofilms, although the growth rates were similar (0.038 to 0.043 h-1). Both biofilm types were resistant to amphotericin B, but daughter cells from iron-limited biofilms were significantly more susceptible to the drug than those from glucose-limited biofilms.

* Corresponding author. Mailing address: Division of Infection and Immunity, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, United Kingdom. Phone: 0141-330-5842. Fax: 0141-330-4600. E-mail: J.Douglas{at}bio.gla.ac.uk.

Antimicrobial Agents and Chemotherapy, August 1998, p. 2146-2149, Vol. 42, No. 8
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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