This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Adrian, P. V.
Right arrow Articles by Amyes, S. G. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Adrian, P. V.
Right arrow Articles by Amyes, S. G. B.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, September 1998, p. 2221-2224, Vol. 42, No. 9
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

New Trimethoprim-Resistant Dihydrofolate Reductase Cassette, dfrXV, Inserted in a Class 1 Integron

Peter V. Adrian,1,* Mignon du Plessis,1 Keith P. Klugman,1 and Sebastian G. B. Amyes2

Department of Medical Microbiology, University of the Witwatersrand, and the South African Institute for Medical Research, Johannesburg, South Africa,1 and Molecular Chemotherapy, Department of Medical Microbiology, University of Edinburgh, Edinburgh, United Kingdom2

Received 6 January 1998/Returned for modification 17 February 1998/Accepted 16 June 1998

The nucleotide sequence of a plasmid-borne trimethoprim resistance gene from a commensal fecal Escherichia coli isolate revealed a new dihydrofolate reductase gene, dfrXV, which occurred as a gene cassette integrated in a site-specific manner in a class 1 integron. The new gene shows 84% nucleotide identity and the predicted protein shows 90% amino acid identity with dfrI and DHFR type I, respectively. Genes for spectinomycin resistance, aadA1 [ant (3'')-Ia], and sulfonamide resistance, sulI, were located downstream of dfrXV in a manner identical to that in pLMO229.

* Corresponding author. Mailing address: Department of Medical Microbiology, SAIMR, P.O. Box 1038, Johannesburg 2000, South Africa. Phone: 27 11 489 9335. Fax: 27 11 489 9332. E-mail: petera{at}mail.saimr.wits.ac.za.

Antimicrobial Agents and Chemotherapy, September 1998, p. 2221-2224, Vol. 42, No. 9
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Sincak, C. A, Schmidt, J. M (2009). Iclaprim, a Novel Diaminopyrimidine for the Treatment of Resistant Gram-Positive Infections. The Annals of Pharmacotherapy 43: 1107-1114 [Abstract] [Full Text]  
  • Ahmed, A. M., Hussein, A. I. A., Shimamoto, T. (2007). Proteus mirabilis clinical isolate harbouring a new variant of Salmonella genomic island 1 containing the multiple antibiotic resistance region. J Antimicrob Chemother 59: 184-190 [Abstract] [Full Text]  
  • Lee, J. C., Oh, J. Y., Cho, J. W., Park, J. C., Kim, J. M., Seol, S. Y., Cho, D. T. (2001). The prevalence of trimethoprim-resistance-conferring dihydrofolate reductase genes in urinary isolates of Escherichia coli in Korea. J Antimicrob Chemother 47: 599-604 [Abstract] [Full Text]  
  • Chang, C.-Y., Chang, L.-L., Chang, Y.-H., Lee, T.-M., Li, Y.-H., Chang, S.-F. (2000). Two new gene cassettes, dfr17 (for trimethoprim resistance) and aadA4 (for spectinomycin/streptomycin resistance), inserted in an Escherichia coli class 1 integron. J Antimicrob Chemother 46: 87-89 [Abstract] [Full Text]  
  • Dalsgaard, A., Forslund, A., Serichantalergs, O., Sandvang, D. (2000). Distribution and Content of Class 1 Integrons in Different Vibrio cholerae O-Serotype Strains Isolated in Thailand. Antimicrob. Agents Chemother. 44: 1315-1321 [Abstract] [Full Text]  
  • Poirel, L., Le Thomas, I., Naas, T., Karim, A., Nordmann, P. (2000). Biochemical Sequence Analyses of GES-1, a Novel Class A Extended-Spectrum beta -Lactamase, and the Class 1 Integron In52 from Klebsiella pneumoniae. Antimicrob. Agents Chemother. 44: 622-632 [Abstract] [Full Text]  
  • Adrian, P. V., Thomson, C. J., Klugman, K. P., Amyes, S. G. B. (2000). New Gene Cassettes for Trimethoprim Resistance, dfr13, and Streptomycin-Spectinomycin Resistance, aadA4, Inserted on a Class 1 Integron. Antimicrob. Agents Chemother. 44: 355-361 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»