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Antimicrobial Agents and Chemotherapy, September 1998, p. 2221-2224, Vol. 42, No. 9
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

New Trimethoprim-Resistant Dihydrofolate Reductase Cassette, dfrXV, Inserted in a Class 1 Integron

Peter V. Adrian,1,* Mignon du Plessis,1 Keith P. Klugman,1 and Sebastian G. B. Amyes2

Department of Medical Microbiology, University of the Witwatersrand, and the South African Institute for Medical Research, Johannesburg, South Africa,1 and Molecular Chemotherapy, Department of Medical Microbiology, University of Edinburgh, Edinburgh, United Kingdom2

Received 6 January 1998/Returned for modification 17 February 1998/Accepted 16 June 1998

The nucleotide sequence of a plasmid-borne trimethoprim resistance gene from a commensal fecal Escherichia coli isolate revealed a new dihydrofolate reductase gene, dfrXV, which occurred as a gene cassette integrated in a site-specific manner in a class 1 integron. The new gene shows 84% nucleotide identity and the predicted protein shows 90% amino acid identity with dfrI and DHFR type I, respectively. Genes for spectinomycin resistance, aadA1 [ant (3'')-Ia], and sulfonamide resistance, sulI, were located downstream of dfrXV in a manner identical to that in pLMO229.

* Corresponding author. Mailing address: Department of Medical Microbiology, SAIMR, P.O. Box 1038, Johannesburg 2000, South Africa. Phone: 27 11 489 9335. Fax: 27 11 489 9332. E-mail: petera{at}mail.saimr.wits.ac.za.

Antimicrobial Agents and Chemotherapy, September 1998, p. 2221-2224, Vol. 42, No. 9
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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