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Antimicrobial Agents and Chemotherapy, September 1998, p. 2221-2224, Vol. 42, No. 9
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
New Trimethoprim-Resistant Dihydrofolate Reductase
Cassette, dfrXV, Inserted in a Class 1 Integron
Peter V.
Adrian,1,*
Mignon
du Plessis,1
Keith P.
Klugman,1 and
Sebastian
G. B.
Amyes2
Department of Medical Microbiology,
University of the Witwatersrand, and the South African Institute
for Medical Research, Johannesburg, South
Africa,1 and
Molecular Chemotherapy,
Department of Medical Microbiology, University of Edinburgh,
Edinburgh, United Kingdom2
Received 6 January 1998/Returned for modification 17 February
1998/Accepted 16 June 1998
The nucleotide sequence of a plasmid-borne trimethoprim resistance
gene from a commensal fecal Escherichia coli isolate
revealed a new dihydrofolate reductase gene, dfrXV, which
occurred as a gene cassette integrated in a site-specific manner
in a class 1 integron. The new gene shows 84% nucleotide identity and
the predicted protein shows 90% amino acid identity with
dfrI and DHFR type I, respectively. Genes for
spectinomycin resistance, aadA1 [ant
(3'')-Ia], and sulfonamide resistance, sulI, were
located downstream of dfrXV in a manner identical to that
in pLMO229.
*
Corresponding author. Mailing address: Department of
Medical Microbiology, SAIMR, P.O. Box 1038, Johannesburg 2000, South Africa. Phone: 27 11 489 9335. Fax: 27 11 489 9332. E-mail:
petera{at}mail.saimr.wits.ac.za.
Antimicrobial Agents and Chemotherapy, September 1998, p. 2221-2224, Vol. 42, No. 9
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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