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Antimicrobial Agents and Chemotherapy, September 1998, p. 2443-2445, Vol. 42, No. 9
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Ser315Thr Substitution in KatG Is Predominant in Genetically Heterogeneous Multidrug-Resistant Mycobacterium tuberculosis Isolates Originating from the St. Petersburg Area in Russia

Harri J. Marttila,1,* Hanna Soini,1 Erkki Eerola,2 Elena Vyshnevskaya,3 Boris I. Vyshnevskiy,3 Tatjana F. Otten,3 Alexandr V. Vasilyef,3 and Matti K. Viljanen1

Mycobacterial Reference Laboratory, National Public Health Institute,1 and Department of Medical Microbiology University of Turku,2 Kiinamyllynkatu 13, 20520 Turku, Finland, and Research Institution of Phthisiopulmonology, St. Petersburg, Russia3

Received 9 April 1998/Returned for modification 6 May 1998/Accepted 29 June 1998

Parts of katG and rpoB from 27 Russian Mycobacterium tuberculosis isolates were sequenced to detect mutations causing resistance to isoniazid (INH) and rifampin (RMP), respectively. All 24 INH-resistant isolates had a mutated katG, and 22 of them (91.7%) carried a mutation coding for a Ser315Thr shift. An rpoB mutation was noted for each of the 21 RMP-resistant isolates, with Ser531Leu being the most prevalent change encoded. Only two isolates had identical IS6110 fingerprints.

* Corresponding author. Mailing address: National Public Health Institute, Department in Turku, Kiinamyllynkatu 13, 20520 Turku, Finland. Phone: 358-2-2519255. Fax: 358-2-2519254. E-mail: harmar{at}utu.fi.

Antimicrobial Agents and Chemotherapy, September 1998, p. 2443-2445, Vol. 42, No. 9
0066-4804/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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