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Antimicrobial Agents and Chemotherapy, October 1999, p. 2400-2403, Vol. 43, No. 10
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Characterization of Mutations in the rpoB Gene in Naturally Rifampin-Resistant Rickettsia Species

Michel Drancourt and Didier Raoult*

Unité des Rickettsies CNRS UPRES-A 6020, Faculté de Médecine, Université de la Méditerranée, 13385 Marseille Cedex 05, France

Received 22 March 1999/Returned for modification 30 June 1999/Accepted 27 July 1999

Rickettsiae are gram-negative, obligately intracellular bacteria responsible for arthropod-borne spotted fevers and typhus. Experimental studies have delineated a cluster of naturally rifampin-resistant spotted fever group species. We sequenced the 4,122- to 4,125-bp RNA polymerase beta -subunit-encoding gene (rpoB) from typhus and spotted fever group representatives and obtained partial sequences for all naturally rifampin-resistant species. A single point mutation resulting in a phenylalanine-to-leucine change at position 973 of the Rickettsia conorii rpoB sequence and present in all the rifampin-resistant species was absent in all the rifampin-susceptible species. rpoB-based phylogenetic relationships among these rickettsial species yielded topologies which were in accordance with previously published phylogenies.

* Corresponding author. Mailing address: Unité des Rickettsies, Faculté de Médecine, 27, Blvd. Jean Moulin, 13385 Marseille Cedex 5, France. Phone: 33.04.91.32.43.75. Fax: 33.04.91.38.77.72. E-mail: Didier.Raoult{at}medecine.univ-mrs.fr.

Antimicrobial Agents and Chemotherapy, October 1999, p. 2400-2403, Vol. 43, No. 10
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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