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Antimicrobial Agents and Chemotherapy, October 1999, p. 2400-2403, Vol. 43, No. 10
Unité des Rickettsies CNRS UPRES-A
6020, Faculté de Médecine, Université de la
Méditerranée, 13385 Marseille Cedex 05, France
Received 22 March 1999/Returned for modification 30 June
1999/Accepted 27 July 1999
Rickettsiae are gram-negative, obligately intracellular bacteria
responsible for arthropod-borne spotted fevers and typhus. Experimental
studies have delineated a cluster of naturally rifampin-resistant spotted fever group species. We sequenced the 4,122- to 4,125-bp RNA
polymerase
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Characterization of Mutations in the
rpoB Gene in Naturally Rifampin-Resistant
Rickettsia Species
-subunit-encoding gene (rpoB) from typhus and spotted fever group representatives and obtained partial sequences for
all naturally rifampin-resistant species. A single point mutation resulting in a phenylalanine-to-leucine change at position 973 of the
Rickettsia conorii rpoB sequence and present in all the rifampin-resistant species was absent in all the rifampin-susceptible species. rpoB-based phylogenetic relationships among these
rickettsial species yielded topologies which were in accordance with
previously published phylogenies.
*
Corresponding author. Mailing address: Unité des
Rickettsies, Faculté de Médecine, 27, Blvd. Jean Moulin,
13385 Marseille Cedex 5, France. Phone: 33.04.91.32.43.75. Fax:
33.04.91.38.77.72. E-mail:
Didier.Raoult{at}medecine.univ-mrs.fr.
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